大鼠尸体细菌演替规律及其在死亡时间推断中的应用(英文)  

Bacterial Succession on Rat Carcasses and Applications for PMI Estimation

作　　者：张琳[1] 郭娟娟[1] 特拉提·赛依提 彭钰龙[1] 谢丹[1] 郭亚东[1] 闫杰[1] 扎拉嘎白乙拉 蔡继峰[1] 

机构地区：[1]中南大学基础医学院法医系,湖南长沙410013 [2]新疆警察学院刑侦系,新疆乌鲁木齐830011

出　　处：《法医学杂志》2016年第1期1-6,共6页Journal of Forensic Medicine

基　　金：supported by grants from the National Natural Science Foundation of China(81373249and 81571855);the Science and Technology Committee of Shanghai Municipality(KF1203);the Fundamental Research Funds for the Central Universities of Central South University(2015zzts276);the Graduate Student Education Innovation Projects of Central South University(160020009)

摘　　要：目的探讨大鼠尸体细菌演替规律并评估其在死亡时间(PMI)推断中的应用价值。方法选取成年雌性SD大鼠,处死后置于纸箱内自然腐败,采集大鼠眼周皮肤、口腔和阴道三个部位的细菌样本,使用纯培养生化方法鉴定菌种,记录菌群构成变化规律。结果大鼠三个部位细菌演替规律显示,葡萄球菌属和奈瑟菌属为死后初期的优势菌属,尤其是金黄色葡萄球菌与乳糖奈瑟菌在死后6 h内相对含量较高。干酪乳杆菌规律地出现在死后第3或第4天,且在死后晚期维持较恒定水平。结论大鼠死后三个部位的正常细菌及腐败细菌呈现一定的演替规律,可用于PMI推断。Objective To investigate the bacterial succession on rat carcasses and to evaluate the use of bacterial succession for postmortem interval （PMI） estimation. Methods Adult female SD rat remains were placed in carton boxes. The bacterial colonization of circumocular skin, mouth and vagina was collected to be identified using culture-dependent biochemical methods. The changes in community composition were regularly documented. Results The bacterial succession in three habitats showed that Staphylococcus and Neisseria were predominated in early PMI, especially Staphylococcus aureus and Neisseria lactamica in 6 hours after death. Lactobacillus casei developed on the 3-4 days regularly, and kept stable at a certain level in late PMI. Conclusion The involvement of normal and putrefactive bacteria in three body habitats of rat remains can be used for PMI estimation.

关 键 词：法医病理学 细菌 死亡时间 大鼠 

分 类 号：R446.1[医药卫生—诊断学]