猪肋软骨和肋骨的ATR-FTIR光谱变化与死亡时间的关系  

Relationship between PMI and ATR-FTIR Spectral Changes in Swine Costal Cartilages and Ribs

作　　者：姚尧[1,2] 王琪[1] 荆小莉[2] 李冰[1] 张胤鸣[1] 王志军[1] 李成志[1] 林汉成[1] 张吉[1] 黄平[3] 王振原[1] 

机构地区：[1]西安交通大学法医学院,陕西西安710061 [2]西安市公安局高新分局,陕西西安710065 [3]司法部司法鉴定科学技术研究所上海市法医学重点实验室,上海200063

出　　处：《法医学杂志》2016年第1期21-25,共5页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(81273339;81273335)

摘　　要：目的应用衰减全反射傅里叶变换红外(attenuated total reflection-Fourier transform infrared,ATRFTIR)光谱技术分析长白猪死后肋软骨和肋骨组织随死亡时间推移的化学降解过程,为死后较长时间段内推断死亡时间提供可行的新途径和方法。方法猪放血处死后取肋软骨和肋骨离体置于20℃环境,后每72 h提取部分组织样本,进行ATR-FTIR光谱分析,并与对应死亡时间点进行相关性分析。结果随死亡时间的延长,肋软骨与肋骨FTIR的主要吸收峰峰位没有发生明显变化,而其部分峰强比出现了不同时序性的变化趋势,并与死亡时间存在良好的相关性,肋软骨较肋骨组织有更好的时序性。结论应用ATRFTIR光谱技术发现死后猪肋软骨和肋骨组织光谱学变化存在一定的规律性,有望成为基于光谱学推断死亡时间的一种新途径。Objective To analyze postmortem chemical changes in Landrace costal cartilages and ribs using attenuated total reflection-Fourier transform infrared （ATR-FT1R） spectroscopy, and to provide a novel technique for estimation of postmortem interval （PMI）. Methods The swines were sacrificed by hemorrhage and their costal cartilages and ribs were kept in 20 ℃. The chemical analysis of the costal cartilages and ribs were performed using ATR-FTIR every 72 h. The correlation between the certain spectral pa- rameters and PMI was also analyzed. The time-dependent changes of costal cartilages were more signifi- cant than ribs. Results There were no obvious changes for the main absorbance bands position, and some absorbance band ratios showed time-dependent changes and significant correlations with the PMI. Conclusion ATR-FTIR has the ability to analyze postmortem chemical changes of the swine costal car- tilages and ribs, and it can be a new method to estimate PMI based on spectroscopy.

关 键 词：法医病理学 谱学 傅里叶变换红外 衰减全反射 肋骨 肋软骨 死亡时间 猪 

分 类 号：R446.129[医药卫生—诊断学]