我国新疆首次检出西伯利亚型蜱传脑炎病毒  

Detection of the Siberian Tick-borne Encephalitis Virus in the Xinjiang Uygur Autonomous Region,northwestern China

作　　者：刘然[1] 张桂林[1] 刘晓明[1] 李裕昌[2] 郑重[1] 孙响[1] 杨银辉[2] 

机构地区：[1]新疆军区疾病预防控制中心,乌鲁木齐830011 [2]军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室,北京100071

出　　处：《病毒学报》2016年第1期26-31,共6页Chinese Journal of Virology

基　　金：国家自然基金项目(81171663;U1303104)资助

摘　　要：对新疆地区蜱传病毒类群进行调查,本研究利用Illumia深度测序技术对新疆全沟硬蜱体内的病毒小RNA分子进行筛查,从提取的蜱小RNA组份中共测得32 631条病毒特异性读序,77条读序与蜱传黄病毒基因组序列匹配,核苷酸数合计约占病毒基因组的3.8%~2.4%,其中32条读序与国内尚未发现的西伯利亚型蜱传脑炎病毒一致。为对上述结果进行确证,利用RT-PCR对此型病毒核酸进行检测,从蜱总RNA中分别扩增获得病毒包膜基因与基因组3′末端片段,测序比对表明上述片段序列与西伯利亚南部检出病毒高度近似,核苷酸与氨基酸似然率分别为97.2%~95.5%与99.4%~98.3%。综上所述,本研究利用基于深度测序的病毒筛查方法,首次从我国新疆蜱传脑炎疫源地检出西伯利亚型病毒,为下一步针对病毒的分离与流行病学调查提供了重要线索。Until the recent emergence/re-emergence of human-pathogenic viruses in ticks,tick-borne viruses have been neglected as causative agents of human disease（particularly in China）.To gain insight into the diversity of tick-borne viruses in Xinjiang Uygur Autonomous Region（northwestern China）,we conducted illumina deep sequencing-based screening for virus-derived small RNAs in field-collected Ixodes persulcatus ticks.We found 32,631 unique virus-matched reads.In particular,77 reads mapped to the tick-borne group within the genus of Flavivirus,and covered 3.8%-2.4% viral genomes.In addition,32 unique reads were specific to the Siberian subtype of tick-borne encephalitis viruses（TBEV-Sib）which have never been reported in Chinese TBE loci.We confirmed the potential existence of TBEV-Sib by amplification（using reverse transcription-polymerase chain reaction）of genomic fragments from the envelope gene or 3′genomic terminus from the pools of examined ticks.Both sequences demonstrated high homology to TBEV-Sib strains attached geographically to southern Siberia with nucleotide identity of 97.2%-95.5% and aminoacid identity of 99.4%-98.3%,respectively.In conclusion,we report,for the first time,detection of TBEV-Sib in the natural TBE loci of China.These novel data may provide genetic information for further isolation and epidemiologic investigation of TBEV-Sib.

关 键 词：蜱传脑炎病毒 西伯利亚型 深度测序 小RNA 新疆 

分 类 号：R373.3[医药卫生—病原生物学]