HPLC测定红凤菜总黄酮含量  

Content of Total Flavonoids in Gynura bicolor Detected with HPLC

作　　者：任冰如[1] 陈剑[1] 吕寒[1] 梁呈元[1] 吴菊兰[1] 刘艳[1] 李维林[1] 

机构地区：[1]江苏省中国科学院植物研究所,江苏南京210014

出　　处：《中国野生植物资源》2016年第1期28-30,34,共4页Chinese Wild Plant Resources

基　　金：江苏省产学研联合创新资金(BY2012213);江苏省科技基础设施建设计划-科技公共服务平台项目(BM2011117)

摘　　要：用70%甲醇浸提红凤菜样品的干燥粉末,将所得浸膏进行酸解,以槲皮素、山柰酚为对照品,用HPLC对酸解液进行测定,经计算得到红凤菜样品的总黄酮含量,为红凤菜的资源利用提供依据。结果表明,红凤菜样品的总黄酮含量为:嫩芽0.0326 mg·g^(-1)DW,嫩叶0.1171 mg·g^(-1)DW,成熟叶片0.1081 mg·g^(-1)DW,整株样品0.1227 mg·g^(-1)DW;所有样品酸解液中的山柰酚含量均高于槲皮素。红凤菜嫩芽的总黄酮含量最低,整株样品的总黄酮含量不低于其他样品;山柰酚是红凤菜总黄酮的主要苷元。The total flavonoids of Gynura bicolor were extracted with 70% methanol, hydrolyzed by acid, and detected with HPLC using quercetin and kaempferol as reference substance. Content of total fla- vonoids in G. bicolor was 0.032 6 mg· g-IDW in apical bud, 0. 117 1 mg·g-i DW in tender leaves, 0. 1081 mg · g-1 DW in mature leaves and 0. 122 7 mg · g-1 DW in the total aerial parts, respectively. Contents of kaempferol were higher than quercetin in all samples. Total flavonoids was lowest in apical bud and higher in the total aerial parts. Kaempferol is the main aglycon of flavonoids in Gynura bicolor.

关 键 词：红凤菜 高效液相法 总黄酮 槲皮素 山柰酚 

分 类 号：R284.1[医药卫生—中药学]