斜带石斑鱼(♀)×鞍带石斑鱼(♂)受精细胞学的组织切片观察  

Study of Fertilization Biology in Epinephelus coioides(♀)×Epinephelus lanceolatus(♂)

作　　者：樊佳伟[1] 吴廷昌[1] 陈猛猛[1] 骆剑[1] 陈国华[1] 

机构地区：[1]海南大学海洋学院热带生物资源教育部重点实验室,中国海南海口570228

出　　处：《生命科学研究》2016年第1期25-28,共4页Life Science Research

基　　金：国家"863"项目(2012AA10A407);海南省自然科学基金项目(313046)

摘　　要：人工诱导雌核发育二倍化的关键是掌握极体排出和卵裂的时机,掌握该规律最直接的方式即进行受精细胞学的研究。以鞍带石斑鱼（Epinephelus lanceolatus）为父本,斜带石斑鱼（Epinephelus coioides）为母本,通过人工授精获得受精卵,收集受精后不同时刻的卵子,经Smith′s液固定、石蜡包埋、切片、苏木精-伊红染色和显微组织观察。结果表明：受精后第30~60 s精子入卵;第3 min精子星光出现;第5 min卵子排出第二极体;第7~15 min雌雄原核相互靠近,并最终融合;第15 min以后合子核开始分裂。鞍带石斑鱼精子进入斜带石斑鱼卵子的时间与多数硬骨鱼类比较没有显著差别;但是,第二极体排出及第一次卵裂的时间较早。研究展示了斜带石斑鱼与鞍带石斑鱼杂交的受精细胞学的基本发育过程,并为后续的人工诱导石斑鱼雌核发育研究提供了理论依据。The key of fish artificial gynogenesis is to know the time of the second polar body release and the first mitosis. Fertilization cytology is the effective method. Crossbreeding between Epinephelus lanceolatus （♂） and Epinephelus coioides （ ♀ ） was conducted by artificial fertilization. The fertilized eggs were fixed in Smith＇s solution at different time points after fertilization, embedded by paraffin, sectioned, stained by H. E. and observed with microscope. The results showed fertilization procedure. The Epinephelus lanceolatus sperm entered the Epinephelus coioides eggs at 30-60 s post-fertilization. Sperm-aster appeared at 3 min post-fertilization. The second polar body released at 5 min post-fertilization. Male pronucleus was formed prior the formation of female pronucleus, and later they got closer to each other at 7-15 min post-fertilization- First karyokinetic division was developed at 15 min post-fertilization- The time of Epinephelus lanceolatus sperm entering the Epinephelus coioides eggs had no significant difference to the other teleost fish; however, the time of the second polar body re- lease and the first mitosis was earlier than the other fish. The results showed basic fertilization procedure between Epinepphelus lanceolatus and Epinephelus coioides, could provide the theory foundation for artificial gynogenesi.

关 键 词：斜带石斑鱼 鞍带石斑鱼 受精细胞学 极体排出 卵裂 

分 类 号：Q954.44[生物学—动物学]