四丁基丙二胺对人前列腺癌PC-3细胞生长、凋亡及迁移能力的影响  

Effects of Polyamine Analogue Tetrabutyl Propanediamine on Proliferation,Apoptosis and Migration of Human PC-3 Prostate Cancer Cells

作　　者：康乐[1] 王凯[2] 王艳林[1] 盛德乔[1] 

机构地区：[1]三峡大学医学院肿瘤微环境与免疫治疗湖北省重点实验室,中国湖北宜昌443002 [2]武汉工程大学化工与制药学院,中国湖北武汉430073

出　　处：《生命科学研究》2016年第1期40-44,49,共6页Life Science Research

基　　金：国家自然科学基金项目(81172788;30973445);湖北省高等学校优秀中青年科技创新团队计划项目(T201203)

摘　　要：多胺类似物干扰正常多胺代谢,耗竭肿瘤快速生长必须的多胺。综合(3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide,MTT)法、流式细胞术、琼脂糖凝胶电泳、细胞划痕和Transwell技术以及Western-blot分析四丁基丙二胺对肿瘤细胞生长、凋亡及侵袭能力的影响。研究发现,四丁基丙二胺可明显抑制人前列腺癌PC-3细胞的生长,抑制效应呈浓度和时间的依赖性。四丁基丙二胺可诱导人前列腺癌PC-3细胞染色体DNA发生片段化降解,降低细胞内凋亡抑制蛋白(B cell lymphoma/lewkmia-2,Bcl-2)水平,但增加促凋亡蛋白细胞色素C在胞浆中的含量,明显抑制细胞周期并降低细胞的转移能力。结果表明四丁基丙二胺可抑制人前列腺癌PC-3细胞增殖和迁移,其机制与干扰细胞周期和诱导细胞凋亡密切相关。Polyamine analogues interfere with the normal metabolism of polyamines, and deplete intracellular polyamines that are necessary for fast-growing tumors. It was performed with MTT, the flow cytometry, the agarose-gel electrophoresis, Western-blot and transwell technique to investigate the effects of polyamine analogue tetrabutyl propanediamine on proliferation, apoptosis and migration of human PC-3 prostate cancer cells. It was showed tetrabutyl propanediamine significantly inhibited proliferation and migration of PC-3 cell and the inhibitory effect was in a time- and dose-dependent manner. After tetrabutyl propanediamine treatment, DNA fragmentation of chromosomal DNA, typical for apoptotic cell, was observed and the contents of anti-apoptotic protein Bcl-2 was significantly deceased, but pro-apoptotic protein cytochrome C was significantly increased in the cytosol. In addition, tetrabutyl propanediamine also induced inhibition of cell cycle and deceased cell migration. As mentioned above, and migration of human prostate cancer PC-3 cells by

关 键 词：多胺 多胺类似物 前列腺癌细胞 细胞凋亡 细胞增殖 细胞迁移 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R329.25[医药卫生—基础医学]