养血解毒药对组合治疗银屑病的分子机制预测  

Prediction of the Molecular Mechanism of Yangxue and Jiedu Herbal Pairs Complex Prescription Treatment for Psoriasis

作　　者：姜春燕[1] 王莒生[1] 杨静[2] 李元文[3] 周冬梅[1] 张苍[1] 孙丽蕴[1] 张广中[1] 

机构地区：[1]首都医科大学附属北京中医医院,北京100010 [2]中国中医科学院医学实验中心,100700 [3]北京中医药大学附属东方医院,北京100078

出　　处：《中国中西医结合皮肤性病学杂志》2016年第1期1-5,共5页Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金面上项目:养血解毒法对斑块型银屑病血燥证微血管形成调控机制研(No.81273766);北京市科学技术委员会"首都临床特色应用研究"专项(No.Z111107058811061)

摘　　要：目的探讨养血解毒药对组合干预银屑病的作用靶点及分子网络机制。方法参考《中药大辞典(第2版)》找到当归、鸡血藤、槐花、土茯苓的化学成分,应用Pub Chem数据库检索这些成分对应的靶蛋白;以"Psoriasis"为关键词,在Pub Med gene数据库中检索银屑病的相关基因;然后将上述数据集导入分子网络分析平台,分别构建中药靶蛋白和银屑病相关基因的分子网络及生物学通路;再通过网络映射、比较,可视化呈现养血药对、解毒药对及它们的组合干预银屑病的作用靶点和分子网络机制。结果养血解毒药对组合针对核因子-κB、细胞外信号调节激酶1/2、泛素和26s蛋白酶体分子靶点,以及通过调控芳香烃受体信号通路、雄激素信号通路、内皮型一氧化氮合酶信号通路和磷脂酰肌醇-3激酶/丝氨酸-苏氨酸蛋白激酶信号通路对银屑病发挥协同效应。结论分子网络分析技术是探索中药治疗疾病靶点及机制的有效方法。Objective To explore acting targets and molecular mechanism of Yangxue and Jiedu herbal pairs complex prescription treating psoriasis. Methods The effective components of Angelica sinensis, caulis Spatholobi, flos sophorae and rhizoma smilacis glabrae were retrievalled from the Grand Dictionary of Chinese Medicine （Second Edition）, the human target proteins of those effective components were retrievalled from PuhChem database, and genes related psoriasis were retrievalled from Pubmed gene database. Then the target proteins and genes were respectively uploaded to Ingenuity Pathways Analysis data analysis platform, constructing the molecular networks and biological pathways of each herb and psoriasis. Finally through the network comparison, acting targets and molecular network mechanism of Yangxue and Jiedu herbal pairs complex prescription were presented visually. Results Some molecules, such as nuclear factor-KB, extracellular signal regulated kinase, ubiquitin and 26s proteasome, might be acting targets of Yangxue and Jiedu herbal pairs complex prescription treating psoriasis. Aryl hydrocarbon receptor signaling, androgen signaling, phosphoinositide-3kinase serine / threonine kinase signaling and endothelial nitric oxide synthase signaling might be the therapeutic effect pathway of Yangxue and Jiedu herbal pairs complex prescription treating psoriasis. Conclusion Network pharmacology theory and method is helpful to predict the molecular mechanism of Chinese herbal medicine treating the disease.

关 键 词：银屑病 养血解毒药对组合 分子机制 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]