点阵激光辅助自体成纤维细胞经皮递送的初步研究  

Transdermal Delivery of Autologous Fibroblast by Fractional Ablative Laser

作　　者：吴红巾 周炳荣[1] 张炜明[1] 张家安[1] 谢淑芬[1] 马立文[1] 张婷[1] 刘娟[1] 王申[1] 栗丹[1] 易飞[1] 陶艳玲[1] 苗颖颖 张丽超[1] 骆丹[1] 

机构地区：[1]南京医科大学第一附属医院,南京210029

出　　处：《中国中西医结合皮肤性病学杂志》2016年第1期6-9,共4页Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine

摘　　要：目的观察点阵激光辅助自体成纤维细胞经皮递送对真皮的长期作用。方法选用健康满月家猪,取耳缘皮肤分离培养成纤维细胞,传至三代后收集。取幼猪胸背部皮肤,随机分成空白对照组,点阵激光组,点阵激光联合细胞组,点阵激光联合磷酸盐缓冲液(PBS)组。3个月后取已处理皮肤活检病理检查,以苏木精和伊红(HE)染色、Masson染色法检测各组胶原纤维分布情况,以实时荧光定量聚合酶链式反应(RT-PCR)检测各组Ⅰ型胶原、Ⅲ型胶原m RNA表达情况。结果 HE染色、Masson染色法显示点阵激光组、点阵激光联合细胞组、点阵激光联合PBS组胶原纤维含量较空白对照组增多,排列更紧密;点阵激光联合细胞组胶原纤维含量增多最高;实时荧光定量检测点阵激光联合细胞组与点阵激光组、空白对照组比较,其Ⅰ型胶原、Ⅲ型胶原表达最高。结论点阵激光辅助自体成纤维细胞经皮递送后产生持续刺激真皮胶原增生作用。Objective To study the effect on dermis with combined treatment of fi＇actional ablative laser and autologous fibroblast. Methods One healthy domestic piglet was selected to establish animal model. Ear skin was isolated a.ld cultured fibroblasts. After the third generation the cells was collected. The abdomens of the piglet randomly divided into four groups： control group, fi＇actional ablative laser group, fractional ablative laser with phosphate buffer solution （PBS） group and combination treatment of fi＇aetional ablative laser amt autologous fibroblast group, Three months later, a biopsy was perrowmed. By hematoxylin-eosin （HE） staining, Masson staining and real-time quantitative polymerase chain reaction, we analyzed collagen fiher arrangement and the mRNA and protein levels of type I collagen, type III collagen. Results According to HE staining and Masson staining, the amount of collagen in the fractional laser group, fractional laser with PBS group and fractional laser with autologous fibroblast group were much more than in the control group, and arranged more closely; fractional laser with autologous fibroblast group increased highest; real-time quantitative polymerase chain reaction showed the same results, the collagen expression levels of type Ⅰ and Ⅲ collagen in the fractional laser with autologous fil）roblast group were highesl. Conclusion Transdermal delivery of autologous fibroblast using a fractional ablative laser can stimulate and increase the synthesis of collagen.

关 键 词：点阵激光 自体成纤维细胞 经皮递送 Ⅰ型胶原 Ⅲ型胶原 

分 类 号：R758.1[医药卫生—皮肤病学与性病学]