碳源及植物生长调节剂对亳芍愈伤组织生长和芍药苷积累的影响  

Effects of Carbon Sources and Plant Growth Regulators on Callus Growth and Paeoniflorin Accumulation of Paeonia lactiflora

作　　者：程孝中[1] 许勇[2] 葛永斌[1] 蒲顺昌[1] 燕傲蕾[1] 张宇[1] 徐娟[1] 

机构地区：[1]亳州师范高等专科学校,安徽亳州236800 [2]安徽省医学科学研究院,安徽合肥230061

出　　处：《安徽农业科学》2016年第3期136-137,140,共3页Journal of Anhui Agricultural Sciences

基　　金：安徽省高校自然科学基金项目(KJ2014A167);亳州师范高等专科学校校级科研项目(BZSZKYXM201304)

摘　　要：[目的]研究不同碳源和植物生长调节剂对亳芍愈伤组织生长和芍药苷积累的影响。[方法]以亳芍根茎为外植体,改变培养基中碳源(蔗糖、果糖、葡糖糖、麦芽糖、可溶性淀粉)和植物生长调节剂种类(6-BA、NAA、2,4-D),考察亳芍愈伤组织增长量和芍药苷积累量。[结果]碳源中,对亳芍愈伤组织的增长量以蔗糖最高,葡糖糖最有利于芍药苷积累;组合使用植物生长调节剂6-BA、NAA和2,4-D比单独使用6-BA更有利于愈伤组织生长和芍药苷积累,但高浓度的2,4-D抑制愈伤组织生长和芍药苷积累。[结论]确定了亳芍愈伤组织生长和芍药苷积累的最佳碳源和植物生长调节剂组合,为葡萄糖30 g/L+6-BA 0.5 mg/L+NAA 0.2 mg/L+2,4-D 0.2 mg/L。[ Objective] To study the influence of different carbon sources and plant growth regulators on callus growth and paeoniflorin accumula- tion of Paeonia lactiflora. [ Method] The callus of Paeonia lactiflora root was used as materials. The callus increment and paeoniflorin accumula- tion was studied by manipulation of different carbon sources （sucrose, glucose, fructose, maltose, soluble starch） and plant growth regulators （6- BA, NAA ,2,4-D） .[ Result ] Sucrose was best for the increment of callus,but glucose was best for the paeoniflorin accumulation. Compared with 6-BA, combined utilization of 6-BA,NAA and 2,4-D was more conductive to increase the callus growth and paeoniflorin accumulation. Otherwise, high concentration of 2,4-D inhibited the callus growth and paeoniflorin accumulation. [ Conclusion ] The optimum carbon sources and plant growth regulators for callus growth and paeoniflorin accumulation was glucose 30 g/L ＋ 6-BA 0.5 mg/L ＋ NAA 0.2 mg/L ＋ 2,4-D 0.2 mg/L.

关 键 词：亳芍 愈伤组织 碳源 植物生长调节剂 芍药苷 

分 类 号：Q567.239[生物学—生物化学] Q813.1