医院制剂伤科灵膏含量测定  

Content Determination of Hospital Medicament Shangkeling Ointment

作　　者：陈曾红 何秀容 李江[1] 

机构地区：[1]贵阳中医学院,贵州贵阳550002 [2]贵州宜民中医骨伤烧伤专科医院,贵州贵阳550000

出　　处：《安徽农业科学》2016年第3期146-148,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]建立伤科灵膏的高效液相色谱法含量测定方法。[方法]采用BP-NH2色谱柱测定伤科灵膏中苦参碱和氧化苦参碱的含量;流动相为乙腈：异丙醇：3%磷酸溶液（72.0∶4.5∶23.5）,检测波长210 nm,流速0.8 m L/min。[结果]该含量测定法分离度较好;苦参碱在0.248~1.160μg范围内呈良好的线性关系,平均加样回收率为97.64%（RSD=1.25%）;氧化苦参碱在0.904~4.068μg范围内呈良好的线性关系,平均加样回收率为97.21%（RSD=1.84%）。[结论]建立的方法简便、可行、灵敏、准确,可以用于伤科灵膏的质量控制。[ Objective ] To establish HPLC content determination method for Shangkeling ointment. [ Method] BP-NH2 chromatographic column was adopted to determine matrine and oxymatrine content in Shangkeling ointment; the mobile phase was acetonitrile： isopropyl alcohol： 3% phosphoric acid solution（72.0： 4.5： 23.5）, detection wavelength was 210 rim, flow rate was 0.8 ml/min. [ Result] The content determination method had a good separation degree; the linear relationship of matrine was good in the range of 0.248 to 1. 160 g, and the average recovery was 97.64% （RSD = 1.25% ） ; the linear relationship of oxymatrine was good in the range of 0.904 -4.068 μg, the average recovery was 97.21% （RSD = 1.84% ）. [ Conclusion] The established method is simple, feasible, sensitive and accurate, which can be used in quality control of Shangkeling ointment.

关 键 词：伤科灵膏 高效液相色谱 含量测定 

分 类 号：S567[农业科学—中草药栽培]