竹节参齐墩果烷皂苷对RAW264-7巨噬细胞SIRT1活性影响及抗炎作用研究  

Anti-inflammatory effect of Chikusetsu oleanane saponin on RAW264.7 cell through regulating SIRT1 activity

作　　者：袁琴[1] 袁丁[1,2] 周志勇[1] 刘朝奇[1] 王婷[1] 向婷婷[1] 张长城[1] 

机构地区：[1]三峡大学医学院国家中医药(肿瘤)管理局三级实验室,湖北宜昌443002 [2]仁和医院,三峡大学第二临床医学院,湖北宜昌443001

出　　处：《中国药理学通报》2016年第3期349-354,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81273895);湖北省自然科学基金重点项目(No 2013CFA014)

摘　　要：目的探讨竹节参齐墩果烷皂苷(chikusetsu oleanane saponin,COS)对脂多糖(lipopolysaccharides,LPS)刺激RAW264.7巨噬细胞的SIRT1活性影响及抗炎作用。方法Griess法测定一氧化氮(NO)释放量;免疫印迹(Western blot)法检测炎性因子肿瘤坏死因子-α(TNF-α)、白介素1β(IL-1β)蛋白表达;免疫荧光分析COS对细胞核因子-κB(NF-κB)和沉默信息调节因子1(silent information regulator1,SIRT1)核转运的作用。结果 COS在25~300 mg·L^(-1)与1 mg·L^(-1)LPS共培养时,对RAW264.7细胞生长无明显影响;与LPS组相比,COS能有效抑制NO释放和抑制TNF-α、IL-1β的分泌;还能抑制NF-κB的核移位,上调SIRT1的表达。结论 COS对LPS刺激的RAW264.7细胞炎症具有保护作用,其保护机制可能是COS上调SIRT1表达,促进NF-κB去乙酰化作用,从而抑制NF-κB的核移位,减少TNF-α、IL-1β等炎症因子的产生。Aim To investigate SIRT1 activity and anti-inflammatory effects of Chikusetsu oleanane saponin(COS) on the RAW264.7 macrophage cells induced by lipopolysaccharide(LPS).Methods The nitric oxide(NO) release was detected with Griess method.Western blot was used to detect the expression of tumor necrosis factor-α(TNF-α),interleukin 1β( IL-1β).Nuclear factor-κB(NF-κB) and silent information adjustment factor 1(SIRT1) were tested by immunofluorescence.Results 1 mg·L^(-1) LPS co-cultured with COS at 25~300 mg·L^(-1) had no significant effect on the growth of RAW264.7 cells.Compared with the LPS group,COS effectively inhibited the NO release and suppressed the expression of TNF-α and IL-1β,and also inhibited the translocation of NF-κB and upregulation of SIRT1.Conclusion COS has protective effects on RAW264.7 cells stimulated by LPS,which may be related to up-regulating the expression of SIRT1 and promoting the deacetylation of NF-κB,thereby inhibiting the translocation of NF-κB and reducing the expression of TNF-α and IL-1β.

关 键 词：竹节参齐墩果烷皂苷 RAW264-7细胞 脂多糖 一氧化氮 TNF-α IL-1Β NF-κB SIRT1 

分 类 号：R284.1[医药卫生—中药学] R329.24[医药卫生—中医学]