HPLC法测定大黄总蒽醌胶囊5种蒽醌苷元及5种游离蒽醌含量  

Determination of 5 Hydrolysis Anthraquinone Glycosides and 5 Free Anthraquinones in Rhubarb Total Anthraquinones Capsule by HPLC

作　　者：吴迪[1] 袁海铭[1] 曾宪仪[1] 李艳[1] 

机构地区：[1]江西省药物研究所,南昌330029

出　　处：《江西中医药大学学报》2016年第1期68-70,共3页Journal of Jiangxi University of Chinese Medicine

摘　　要：目的:建立大黄总蒽醌胶囊中5种蒽醌苷元及5种游离蒽醌的高效液相色谱法含量测定方法。方法:采用Alltima C18柱,(4.6mm×250mm,5μm)色谱柱,流动相:甲醇-0.1%磷酸溶液(85∶15),检测波长:440nm。结果:芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚等5种游离蒽醌线性关系良好,水解后5种蒽醌苷元及总游离蒽醌回收率为100.54%和101.58%,RSD分别为1.4%和1.1%。结论:本方法操作快速、准确,可作为大黄总蒽醌胶囊中蒽醌的质量控制方法。Objective： To establish an HPLC method for the determination of 5 hydrolysis anthraquinone glycosides and 5 free anthraquinones in rhubarb total anthraquinones capsule by HPLC. Method： Alltima C18column（ 4. 6mm × 200 mm,5μm） was used in HPLC with mobile phase methanol-0. 1% phosphoric acid solutions（ 85 ∶ 15）,the detection wavelength at 440 nm. Results： the calibration curve was good for the aloe-emodin,rhein,emodin,chrysophanol,physcione content. The average recovery ratio of 5 hydrolysis anthraquinone glycosides and 5 free anthraquinones were 100. 54% and 101. 58%. The relative standard deviation were 1. 4% and 1. 1%. Conclusion： This method is rapid and accurate. It can be used for quality control on anthraquinones of Rhubarb total anthraquinones capsule.

关 键 词：大黄总蒽醌胶囊 5种蒽醌苷元 游离蒽醌 总蒽醌 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]