不同强度运动联合中药对葡萄糖耐量减退大鼠血管内皮功能的影响  

Effects of Sports with Different Strength Combined with Traditional Chinese Medicine on Function of Vascular Endothelium of IGT Rats

作　　者：倪英群[1] 方朝晖[1] 

机构地区：[1]国家中医临床研究糖尿病基地、安徽中医药大学第一附属医院,安徽合肥230031

出　　处：《中国中医药信息杂志》2016年第4期54-57,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家科技重大专项-重大新药创制(2010ZX09102);国家中医临床研究基地业务建设科研专项(JDZX201201);国家中医药管理局中医药重点学科(20091221);国家中医药管理局国家基本公共卫生服务项目(20131012);安徽省教育厅高校自然科学研究项目(KJ2015A136)

摘　　要：目的观察不同强度运动联合中药对葡萄糖耐量减退(IGT)大鼠过氧化物酶体增殖活化受体γ辅助活化因子1α(PGC-1α)的表达及血管内皮功能的影响,探讨不同强度运动与中药联合的协同作用及其机制。方法健康雄性SD大鼠70只,随机取10只为空白组予以常规饲料喂养,其余60只大鼠予以高糖高脂饲料喂养进行造模。将成模大鼠随机分为模型组、单纯中药组(中药组)、低强度运动+中药组(低强度组)、中强度运动+中药组(中强度组)、高强度运动+中药组(高强度组),每组10只。空白组、模型组、中药组每日自由活动,运动各组进行不同强度跑台运动,同时给予丹蛭降糖胶囊灌胃。比较干预前后一氧化氮(NO)、内皮型一氧化氮合酶(e NOS)、血管性假血友病因子(v WF)和血浆内皮微粒(EMP)变化;检测各组PGC-1α水平。结果与空白组比较,模型组NO、e NOS、PGC-1α水平下降,v WF、EMP水平升高(P<0.05);与模型组比较,各干预组NO、e NOS、PGC-1α水平升高,v WF、EMP水平降低(P<0.05,P<0.01)。结论中等强度运动联合丹蛭降糖胶囊能有效改善血管内皮功能。Objective To observe the effects of sports with different strength combined with traditional Chinese medicine on expression of PGC-1α and function of vascular endothelium of IGT rats; To discuss the synergistic action and its mechanism. Methods Seventy healthy male SD rats were randomly divided into blank group(10 rats), which were given regular diet, and the other 60 rats were given high-glucose and high-lipoid diet to establish the IGT model rats, which were then randomly divided into 5 groups, including IGT model group(model group), simple Chinese medicine group(Chinese medicine group), low strength sports+Chinese medicine(low strength group), middle strength+Chinese medicine(middle strength group), and high strength sports+Chinese medicine groups(high strength group), 10 rats in each group. Rats in blank group, model group and Chinese medicine group exercised freely. While rats in different sport strength groups conducted treadmill exercise with different strength, and were given Danzhi Jiangtang Capsules for gavage. Changes of NO, e NOS, v WF and EMP before and after the treatment were compared,the PGC-1α level was detected. Results Compared with the blank group, levels of NO, e NOS and PGC-1α decreased,while the levels of v WF and EMP increased in model group(P<0.05). Compared with the model group, levels of NO,e NOS and PGC-1α increased in all treatment groups, while the levels of v WF and EMP decreased(P<0.05, P<0.01).Conclusion Middle strength sports combined with Danzhi Jiangtang Capsules may effectively improve function of vascular endothelium.

关 键 词：不同强度运动 葡萄糖耐量减退 过氧化物酶体增殖活化受体γ辅助活化因子1α 中药 大鼠 

分 类 号：R285.5[医药卫生—中药学]