钩藤提取物对MPTP诱导帕金森病模型小鼠神经元的影响  

Effects of Uncariae Ramulus Cum Uncis Extract on Neurons of MPTP-induced PD Model Mice

作　　者：卢芳[1] 井月娥[1] 任燕冬[2] 张淑香[1] 刘树民[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]佳木斯医学院,黑龙江佳木斯154000

出　　处：《中国中医药信息杂志》2016年第4期57-60,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金青年基金(81303249);中国博士后科学基金(2013T60398);博士后研究人员落户黑龙江科研启动资助基金(LBH-Q13160);黑龙江中医药大学科研基金(2013jc01)

摘　　要：目的探讨钩藤提取物对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导帕金森病(PD)模型小鼠神经元保护的作用机制。方法将C57BL/6小鼠随机分为对照组、模型组、钩藤组及美多芭组,腹腔注射MPTP法制备PD小鼠模型。行为学测试小鼠抓握和肢体运动协调能力;生化法测定小鼠中脑黑质超氧化物歧化酶(SOD)、丙二醛(MDA)及谷胱甘肽-过氧化物酶(GSH-Px)水平,酶联免疫法测定白细胞介素(IL)-1β、IL-6的活性。结果与对照组比较,模型组小鼠自主活动次数减少、爬杆时间延长(P<0.05),脑组织SOD、GSH-Px酶活性明显下降,MDA、IL-1β、IL-6含量明显上升(P<0.05,P<0.01);与模型组比较,钩藤组和美多芭组小鼠自主活动次数增多、爬杆时间缩短(P<0.05),脑组织SOD、GSH-Px酶活性明显上升,MDA、IL-1β及IL-6含量明显下降(P<0.05,P<0.01)。结论钩藤提取物对PD模型小鼠的治疗作用可能是通过清除氧自由基、提高机体抗氧化能力和抑制炎症反应,从而减少神经元的凋亡实现的。ObjectiveTo study the neuron protection mechanism of Uncariae Ramulus Cum Uncis extract for the MPTP-induced PD mice.MethodsC57BL/6 mice were randomly divided into control group, model group, Uncariae Ramulus Cum Uncis extract group and madopar group, and injected with MPTP in abdominal cavity. Behavior test was used to detect grabbing capacity and body movement coordination capacity: three biochemical indexes (SOD, MDA, GSH-Px) were detected by biochemical process and the activity of two immune enzyme-linked indexes (IL-1β, IL-6) were detected by enzymelinked immunosorbent assay.ResultsCompared with the control group, the autonomic activity numbers of mice increased and climbing pole time decreased in the model group (P<0.05), enzymatic activity of SOD and GSH-Px decreased significantly and the contents of MDA, IL-1β and IL-6 increased obviously (P<0.01). Compared with model group, the autonomic activity numbers of mice increased and climbing pole time decreased in the Uncariae Ramulus Cum Uncis extract group and madopar group (P<0.05). Enzymatic activity of SOD and GSH-Px increased and the contents of MDA and IL-1β and IL-6 decreased (P<0.05,P<0.01).ConclusionUncariae Ramulus Cum Uncis extract can reduce neuronic apoptosis PD mice, whose therapeutic action may be realized through eliminating oxygen free radicals, improving oxidation resistance and reducing inflammatory reactions.

关 键 词：帕金森病 钩藤提取物 神经保护 小鼠 

分 类 号：R285.5[医药卫生—中药学]