铁棒锤不定根诱导及培养的初步研究  

Preliminary Study on Adventitious Root Induction and Culturing of Aconitum Pendulum Busch

作　　者：陈红刚[1,2] 杜弢[1,2] 何佳研 张鑫 杨晓霞 

机构地区：[1]甘肃省高校中(藏)药化学质量研究省级重点实验室,甘肃兰州730000 [2]甘肃中医药大学药用植物遗传育种研究所,甘肃兰州730000

出　　处：《中国中医药信息杂志》2016年第4期80-83,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：甘肃省青年科技基金计划(1308RJYA026);甘肃省高校中(藏)药化学与质量研究省级重点实验室开放基金项目(zzy-2012-03);兰州市科技计划项目(2013-3-115)

摘　　要：目的建立铁棒锤不定根的诱导及增殖培养体系。方法通过组织培养的方法,考察外植体类型及植物生长物质对铁棒锤不定根的诱导及增殖情况的影响。结果不定根诱导能力依次为根>茎>叶,2.5 mg/L吲哚丁酸有利于铁棒锤不定根的诱导及增殖。结论本研究初步建立了铁棒锤不定根的诱导及增殖培养体系,为铁棒锤深层次的资源开发及利用提供了一种新途径。Objective To establish the adventitious root induction and culture system ofAconitum pendulum Busch.Methods The influence of different explants and plant growth substances on adventitious induction proliferation ofAconitum pendulumBusch were investigated through tissue culturing.Results Explants induction ability was: root>stem>leaf. 2.5 mg/L IBA was in favor of induction and multiplication adventitious root ofAconitum pendulumBusch.ConclusionThe culture system of induction and proliferation adventitious roots ofAconitum pendulum Busch was preliminary established, which provides a new way for resource exploitation and utilization of Aconitum pendulum Busch.

关 键 词：铁棒锤 不定根 诱导 增殖 

分 类 号：R282.2[医药卫生—中药学]