多指标综合评分法优选三拗汤提取工艺研究  

Study on Extracting Process of San Ao Decoction by Multi-indexes Grading Method

作　　者：张金花[1] 刘陶世[1] 程建明[1] 毕肖林[1] 钱海峰[1] 陈冬冬[1] 

机构地区：[1]南京中医药大学药学院,江苏南京210046

出　　处：《中国中医药信息杂志》2016年第4期91-94,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81403114);江苏高校优势学科建设工程资助项目(2014年)

摘　　要：目的采用高效液相色谱法同时测定三拗汤提取液中盐酸麻黄碱、盐酸伪麻黄碱、苦杏仁苷和甘草酸4种有效成分的含量,优选三拗汤最佳提取工艺条件。方法采用正交试验,以加水量、煎煮时间和煎煮次数为考察因素,以盐酸麻黄碱、盐酸伪麻黄碱、苦杏仁苷和甘草酸转移率以及出膏率为考察指标,进行多指标综合评分。结果三拗汤提取影响因素依次为煎煮次数>煎煮时间>加水量。最佳提取工艺条件为加10倍量水,煎煮3次,每次1 h。结论本研究优选得到的提取工艺稳定可行。Objective To simultaneously determinate the contents of four active ingredients (Ephedrine Hydrochloride, Pseudoephedrine Hydrochloride, Amygdalin and Glycyrrhizinate) ofSan Ao Decoction by HPLC; To optimize the extracting process ofSan Ao Decoction.Methods The orthogonal test was employed. Volume of water, time of extraction and times of extraction were set as investigation factors; the transport rates of Ephedrine Hydrochloride, Pseudoephedrine Hydrochloride, Amygdalin and Glycyrrhizinate and the extraction rates were set as investigation indexes to conduct multi-indexes grading.Results The influence factors of the extraction ofSao Ao Decoction were the times of extraction > decoction time > the volume of water. The best extraction condition was: ten fold water; extract for three times; 1 h for each time.Conclusion The optimized extraction process is stable and feasible.

关 键 词：三拗汤 正交试验 提取工艺 

分 类 号：R283.5[医药卫生—中药学]