响应曲面法优化光合细菌PB-Z的产氢条件  

Optimization of Hydrogen Production for Photosynthetic Bacteria by Response Surface Methodology

作　　者：张乐[1,2] 吕永康[2] 刘玉香[3] 孙明星[1,2] 

机构地区：[1]太原理工大学化学化工学院 [2]太原理工大学煤科学与技术教育部和山西省重点实验室 [3]太原理工大学环境科学与工程学院,山西太原030024

出　　处：《环境科学与技术》2016年第2期6-10,138,共6页Environmental Science & Technology

基　　金：山西省国际合作计划项目(2013081020)

摘　　要：为探究光合细菌PB-Z产氢的最适产氢条件,在单因素实验的基础上,以镁离子浓度、p H、反应时间为实验因素,以光合细菌PB-Z产氢量为响应值,采用响应面分析法对实验结果进行模拟及分析,建立了产氢量与3个自变量关系的二次多项式数学模型。结果表明,镁离子浓度、p H值、反应时间与产氢量存在显著的相关性。确定光合细菌PB-Z的最佳产氢条件为:镁离子浓度4.77 mmol/L,初始p H 7.05,反应时间77.5 h。在此实验条件下,产氢量为689.3 m L/L-培养基,与预测值相比偏差仅4.8%。说明响应曲面法优化光合细菌PB-Z的产氢条件是可行的。The most suitable hydrogen production conditions of photosynthetic bacteria were studied for replication.On the basis of single factor experiment, magnesium ion concentrations, initial p H value, reaction time were selected as independent variables, and the hydrogen yield as the response value, experimental results were simulated and analyzed by response surface methodology. A quadratic polynomial equation was established for describing the relationship between hydrogen yield and three independent variables. Response surface analysis results demonstrate that the selected factors had significant correlation with the hydrogen yield. The optimum conditions of hydrogen yield were determined to be magnesium ion concentration 4.77mmol/L, initial p H value 7.05, reaction time 77.5 h. Under these conditions, the hydrogen production of photosynthetic bacteria was 689.3 ml/L-culture, the value agreed with the predicted value with 4.8% deviation. The results showed that the experimental value was comparable to model prediction and the method was practical.

关 键 词：光合细菌 镁离子 PH 反应时间 响应曲面法 

分 类 号：X703.1[环境科学与工程—环境工程]