豌豆凝集素化载多西他赛聚羟基丁酸酯纳米粒对人乳腺癌MCF-7细胞的细胞摄取实验及抑制作用  

Uptake and inhibition effects of pisum sativum agglutinin-anchored docetaxel poly(β-hydroxybutyrate)nanoparticles on human breast cancer MCF-7cells

作　　者：李波[1] 朱明洁[2] 段友容[2] 徐希明[1] 李凤前 

机构地区：[1]江苏大学药学院药剂学教研室,江苏镇江212013 [2]上海市肿瘤研究所纳米靶向治疗研究组,上海200032 [3]上海市徐汇区大华医院,上海200237

出　　处：《药学服务与研究》2016年第1期6-10,共5页Pharmaceutical Care and Research

基　　金：国家自然科学基金(81172989);上海市科学技术委员会基础研究领域项目(13NM1400702)

摘　　要：目的:考察豌豆凝集素化载多西他赛聚羟基丁酸酯纳米粒[pisum sativum agglutinin-anchored docetaxel poly(β-hydroxybutyrate)nanoparticles,PSA-DTX-PHB NPs]对人乳腺癌MCF-7细胞的摄取情况和抑制作用。方法:通过激光共聚焦显微镜观察纳米粒与MCF-7细胞共孵育4h后纳米粒的摄取情况。用MTT法测定纳米粒与MCF-7细胞共孵育24、48和72h后MCF-7细胞的存活率。结果:激光共聚焦显微镜观察显示,豌豆凝集素的修饰能够显著提高MCF-7细胞对纳米粒的摄取量。MTT法发现,载体材料聚羟基丁酸酯对MCF-7细胞无明显细胞毒性。72h时,DTX、DTX-PHB NPs和PSA-DTX-PHB NPs的半数抑制浓度(IC50)分别为(70.24±1.85)、(56.93±1.76)和(9.37±0.97)μg/ml。结论:与空白纳米粒相比,豌豆凝集素化修饰能够显著提高多西他赛纳米粒向MCF-7细胞的递药效率,从而增加其体外细胞毒性。Objective：To investigate the uptake and inhibition effects of pisum sativum agglutinin-anchored docetaxel poly（β-hydroxybutyrate）nanoparticles（PSA-DTX-PHB NPs）on human breast cancer MCF-7cells.Methods：The uptake of the nanoparticles was measured by confocal laser scanning microscopy after incubation with MCF-7cells for 4h.MTT assay was applied to investigate cell viability of MCF-7cells after incubation with the nanoparticles for 24,48 and 72h,respectively.Results：Through observation by confocal laser scanning microscopy,it was indicated that the modification of PSA could significantly improve the cellular uptake of the nanoparticles.MTT assey showed that the carrier material PHB had no obvious cytotoxicity to MCF-7cells.The inhibitory concentration（IC50）of DTX,DTX-PHB NPs and PSA-DTX-PHB NPs were respectively（70.24±1.85）,（56.93±1.76）and（9.37±0.97）μg/ml after incubation with MCF-7cells for 72 h.Conclusion：Compared with the blank nanoparticles,the PSA modification could significantly improve the drug delivery rate of DTX NPs to MCF-7cells,thus enhancing its in vitro cytotoxicity.

关 键 词：多西他赛 豌豆凝集素 细胞毒性 聚羟基丁酸酯纳米粒 MTT法 

分 类 号：R944.9[医药卫生—药剂学] R979.1[医药卫生—药学]