黑参中人参皂苷的大孔吸附树脂纯化工艺研究  

Study on Purification of Total Saponins from Black Ginseng by Macroporous Resins

作　　者：袁媛[1] 

机构地区：[1]辽宁省中医药研究院,辽宁沈阳110034

出　　处：《亚太传统医药》2016年第5期41-43,共3页Asia-Pacific Traditional Medicine

基　　金：辽宁省博士启动基金(20121135)

摘　　要：目的:以人参新炮制品黑参为原料,研究大孔吸附树脂纯化人参皂苷的工艺条件。方法:以人参皂苷含量为指标,比较不同型号树脂和不同工艺条件对人参皂苷的分离纯化能力。结果:确定黑参水提液的最佳大孔树脂纯化工艺条件为:选用RE-A树脂,流速为3mL/min,溶媒为70%的乙醇洗脱溶媒量为100mL,最佳吸附时间为30min。结论:实验为人参皂苷的中药制剂的制备提供依据。Objective： To study the optimum methods of extracting and refining ginsenosides from black ginseng. Methods：The a bility of extracting and refining ginsenosides was compared in diffrent types of macroporus resin and different technological conditions with total amount of ginsenosides as index. Results ： The macroporous resin was best for separating and purifying total saponins in the following technological conditions：RE-A macroreticular resin the flow rate was 2mL/min, the eluting reagent was 70 % ethanol and eluting velocity was 100mL, time was 30min. Conclusion： The technological condition will provide bases for the preparation technique of Traditional Chinese Medicine containing ginsenosides.

关 键 词：黑参 大孔吸附树脂 人参皂苷 纯化 

分 类 号：R282[医药卫生—中药学]