不同性别冠状动脉粥样硬化患者维生素D受体基因多态性特点及临床特征  

The vitamin D receptor gene polymorphism characteristics and clinical features in women and man with coronary atherosclerosis

作　　者：何丽娜[1] 王梦洪[1] 胡小亮[1] 文通[1] 张智亮[1] 

机构地区：[1]南昌大学第一附属医院心内科,南昌330006

出　　处：《临床心血管病杂志》2016年第2期160-165,共6页Journal of Clinical Cardiology

摘　　要：目的:探讨中国南方汉族不同性别冠状动脉粥样硬化患者维生素D受体基因多态性的分布特点及临床特点。方法:应用Sanger法测序检测242例冠状动脉粥样硬化患者维生素D受体Tru9Ⅰ(rs757343),ApaⅠ(rs7975232),TaqⅠ(rs731236)和FokⅠ(rs2228570)基因型,其中男171例,女71例;比较其在不同性别间的分布差异,及不同性别冠心病患者间各临床及生化参数的差异。结果:维生素D受体基因FokⅠ酶切位点:两组AA基因型、AG基因型、GG基因型频率分别为:男性22.2%、48.0%和29.8%,女性:23.9%、46.5%和29.6%,差异无统计学意义(P=0.771、0.834、0.969),等位基因A/G分布频率差异无统计学意义(P=0.912);Tru9Ⅰ酶切位点:AG基因型及GG基因型差异无统计学意义(P=0.879,0.281),AA基因型频率存在性别差异(P=0.037),等位基因A/G分布频率无差异(P=0.109);ApaⅠ酶切位点的不同性别组比较:AC基因型及CC基因型不存在统计学差异(P=0.267、0.993),AA基因型频率存在性别差异(P=0.047),等位基因A/C分布频率无差异(P=0.404);TaqⅠ酶切位点不同性别组比较:TT基因型、CT基因型不存在统计学差异(P=0.903),CC基因型均未测得,无法比较,等位基因T/C分布频率无差异(P=0.906)。进一步比较两组间各参数,结果发现两组间年龄构成差异无统计学意义的情况下,女性患者较男性患者有较低的肌酐、空腹血糖、冠脉积分和较高的总胆固醇、高密度脂蛋白、低密度脂蛋白,且存在统计学差异(P值分别为0.001,0.044,0.002,0.002,0.002,0.049)。采用分层分析示女性ApaⅠ不同基因型总胆固醇及低密度脂蛋白水平存在统计学差异(P=0.035,0.027)。结论:中国南方汉族人群冠状动脉粥样硬化中不同性别间维生素D受体基因FokⅠ、TaqⅠ酶切位点多态性分布频率无明显差异,Tru9Ⅰ、ApaⅠ酶切位点多态性分布频率存在统计学差异;两者间存在不同的临床特点,女性患者合并血脂代谢异常较其他更为�Objective：To detect the The Vitamin D Receptor Gene Polymorphism Characteristics and Clinical Features with Coronary atherosclerosis in Women and Man of Han nationalities in South China.Method：By using Sanger sequencing analysis method,VDR gene types in 242 CAS patients（man 171,female 71）were detected,and the frequencies of gene types and clinical features with CHD in female group and man group were compared.Result：The frequencies of AA,AG and GG types of Fok Ⅰ polymorphism in man group and female group were22.2%,48.0% and 29.8% vs 23.9%,46.5% and 29.6%,respectively no significant differences in the two groups（P=0.771,0.834,0.969）.Allen gene A/G＇s frequencies have no differences（P=0.912）;The frequencies of AG and GG types of Tru9Ⅰ polymorphism in man group and female group were no significant differences（P=0.879,0.281）,The frequenc of AA type of Tru9 Ⅰ polymorphism in different gender groups was significant difference（P=0.037）,allen gene A/G＇s frequencies have no differences（P=0.109）;The frequencies of AC and CC types of ApaⅠ polymorphism were no significant differences in man group and female group（P=0.267,0.993）,The frequenc of AA type of Apa Ⅰ polymorphism in different gender groups was significant difference（P=0.047）,allen gene A/C＇s frequencies have no differences（P=0.404）;The frequencies of TT and CT types of TaqⅠ polymorphism in man group and female group were no significant differences in the two groups（P =0.903）,allen gene T/C＇s frequencies have no differences（P=0.906）.Further comparing the parameters,age have no statistical significance between the two groups（P=0.14）.The creatinine,fasting blood glucose and coronary integral were lower,but the total cholesterol,high density lipoprotein cholesterol（HDL-c）and low density lipoprotein cholesterol（LDL-c）were higher in female group,and the statistical difference（Pvalue were 0.001,0.001,0.044,0.002,0.002,0.049）.The lipid levels in women with difference ApaⅠ genotype was sig

关 键 词：性别 维生素D受体 冠状动脉粥样硬化 基因多态性 

分 类 号：R543.5[医药卫生—心血管疾病]