延龄草根及根茎的化学成分研究(Ⅴ)  

Study on Chemical Components in the Roots and Rhizome of Trillium tschonoskii(Ⅴ)

作　　者：左月明[1] 徐元利[1] 张忠立[1] 刘电航 蔡妙婷[1] 

机构地区：[1]江西中医药大学药学院,江西南昌330006

出　　处：《时珍国医国药》2016年第2期260-263,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金项目(No.81260631);江西省研究生创新专项基金(No.YC2014-S293)

摘　　要：目的研究延龄草Trillium tschonoskii Maxim.根及根茎中的化学成分。方法采用硅胶、聚酰胺、反相硅胶C18、Sephadex LH-20柱色谱和制备高效液相等对其85%甲醇洗脱部位进行分离纯化。应用质谱、一维及二维核磁等波谱学方法确定化合物的结构。结果从延龄草的85%甲醇洗脱部位中分离并鉴定9个化合物的结构,分别为26-O-β-D-吡喃葡萄糖基-（25s）-5α-呋甾烷-△20（22）-烯-3β,26-二醇-3-O-α-L-吡喃鼠李糖基（1→4）-[α-L-吡喃鼠李糖基（1→2）]-β-D-吡喃葡萄糖苷（1）;26-O-β-D-吡喃葡萄糖基-（25s）-5α-呋甾烷-△20（22）-烯-3β,26-二醇-3-O-α-L-吡喃鼠李糖基（1→4）-β-D-吡喃葡萄糖苷（2）;26-O-β-D-吡喃葡萄糖基-（25s）-5α-呋甾烷-△5（6）,20（22）-二烯-3β,26-二醇-3-O-α-L-吡喃鼠李糖基（1→4）-β-D-吡喃葡萄糖苷（3）;deoxytrillenoside A（4）;24-acetyl-deoxytrillenoside A（5）;24-methoxy-trillenoside A（6）;trillenoside B（7）;24-acetyl-trillenoside B（8）;24-acetyl-deoxytrillenoside B（9）。结论化合物1~3首次从该属植物中分离得到,化合物5、6、8和9首次从该种植物中分离得到。Objective To investigate the chemical constituents in the roots and rhizome of Trillium tschonoskii Maxim. Methods The 85% methanol elution parts from the roots and rhizome of Trillium tschonoskii Maxim were separated and purified by polyamide,silica gel,RP- C18,Sephadex LH- 20 column chromatography and the preparation of high performance liquid,et al.Chemical structures were identified by MS,1D and 2D NMR experiments. Results Nine compounds were isolated and identified from the 85% methanol elution parts of Trillium tschonoskii Maxim as 26- O- β- D- glucopyranosyl-（ 25s）- 5α- furostan-△20（ 22）- ene- 3β,26- glycol- 3- O- α- L- rhamnopyranosyl-（ 1 →4）- [α- L- rhamnopyranosyl（ 1 →2） ]- β- D-glucopyranoside（ 1）,26- O- β- D- glucopyranosyl-（ 25s）- 5α- furostan- △20（ 22）- ene- 3β,26- glycol- 3- O- α- L- rhamnopyranosyl-（ 1→4）- β- D- glucopyranoside（ 2）,26- O- β- D- glucopyranosyl-（ 25s）- 5α- furostan- △5（ 6）,20（ 22）- diene- 3β,26- glycol- 3- O- α- L- rhamnopyranosyl-（ 1 →4）- β- D- glucopyranoside（ 3）,deoxytrillenoside A（ 4）,24- acetyl- deoxytrillenoside A（ 5）,24- methoxy- trillenoside A（ 6）,trillenoside B（ 7）,24- acetyl- trillenoside B（ 8）,24- acetyl- deoxytrillenoside B（ 9）. Conclusion Compounds 1 ~ 3 for the first time isolated from this genus,compounds5,6,8 and 9 for the first time isolated from this plant.

关 键 词：延龄草 甾体皂苷 化学成分 

分 类 号：R284.2[医药卫生—中药学]