基于psbA-trnH基因序列对中药材茜草的分子鉴定  

Molecular authentication of Rubiae Radix Et Rhizoma based on psbA-trnH Gene Sequences

作　　者：夏至[1] 王璐静[1] 李贺敏[1] 张红瑞[1] 高致明[1] 

机构地区：[1]河南农业大学农学院中药材工程技术研究中心,河南郑州450002

出　　处：《时珍国医国药》2016年第2期374-376,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金联合基金项目(U1404302)

摘　　要：目的通过对中药材茜草及其近缘种（易混品）的psb A-trn H基因序列进行分析,为中药材茜草的分子鉴定提供依据。方法提取中药材茜草及其近缘种的DNA,对叶绿体基因psb A-trn H序列扩增和测序,计算物种种内、种间Kimura 2-parameter（K2P）遗传距离。采用最近距离、相似性搜索、构建Neighbor-joining（NJ）系统聚类树3种方法进行鉴定分析。结果 psb A-trn H分子系统树支持中药材茜草不同居群样本聚为一单系分支,支持率为65%;中药材茜草与其近缘种psb A-trn H序列的种间遗传距离为0.00751~0.19338,远大于中药材茜草种内遗传距离0~0.00372。结论叶绿体基因psb A-trn H序列可以快速准确鉴别中药材茜草。Objective To provide the evidence for the molecular authentication of Rubiae Radix Et Rhizoma through psb A- trn H analysis on Rubia cordifolia and its relative species. Methods Total genomic DNA was isolated from R. cordifolia and its relative species. Chloroplast gene psb A- trn H sequences were amplified and sequenced. The Kimura 2- Parameter（ K2P） distances were calculated. Molecular authentication analysis were performed using Nearest Distance,BLAST1 and Neighbor- joining（ NJ）methods. Results The NJ tree（ psb A- trn H data） indicate that different populations of R. cordifolia form a monophyletic clade[Bootstrap（ BS） = 65%]. The inter- specific genetic distances（ psb A- trn H data） between R. cordifolia and its relative species were 0. 00751 ~ 0. 19338,which were far higher than intra- specific genetic distances of R. cordifolia（ 0 ~ 0. 00372）. Conclusion The psb A- trn H is an efficient barcode for fast and accurate identification of Rubiae Radix Et Rhizoma.

关 键 词：PSBA-TRNH 茜草 分子鉴定 NJ系统树 

分 类 号：R284.1[医药卫生—中药学]