黄芩水煎液中黄芩苷的HPLC测定及其抗内毒素作用  

Determination of Baicalin Component from Water Decoction of Scutellariae baicalensis by HPLC and Study on Anti-endotoxin Effects of Baicalin

作　　者：杨舒[1] 张倩[1] 冯波[1] 王明明[1] 穆祥[1] 

机构地区：[1]北京农学院兽医学(中医药)北京市重点实验室,北京102206

出　　处：《动物医学进展》2016年第3期47-51,共5页Progress In Veterinary Medicine

基　　金：国家现代农业科技成果惠民科技示范工程项目(Z121100007412004);"十二五"国家支撑计划(2011BAD34B03-5);国家自然科学基金项目(31272144)

摘　　要：通过HPLC法分析黄芩水煎液中黄芩苷含量,采用显色基质法鲎试验进行相关单体成分的抗内毒素定量测定。结果表明,黄芩水煎液中含量最多的单体成分为黄芩苷,显色基质法鲎试验结果表明,不同浓度(50、25、12.5、6.25、3.125μg/mL)的黄芩苷溶液对内毒素的破坏率分别为45.08%、54.39%、57.45%、61.78%和82.80%,其破坏率呈浓度依赖性,可知黄芩水煎液中含量最多的成分黄芩苷具有灭活内毒素的作用。The components of baicalin from the water decoction of Scutellariae baicalensis were determined by HPLC,the limulus test was adopted to make a quantitative determination of the related monomer components.The results of HPLC showed that the most component of the water decoction of Scutellariae baicalensisis baicalin.The result of the limulus test showed that different concentrations of baicalin（50,25,12.5,6.25,3.125μg/mL）had different destruction percentages of 45.08%,54.39%,57.45%,61.78%and 82.80% to endotoxixin,respectively,its destruction percentage by baicalin was in a dose-dependent manner.Then the major components of baicalin from the water decoction of Scutellariae baicalensishad anti-endotoxin effects.

关 键 词：黄芩水煎液 黄芩苷 高效液相色谱 抗内毒素 

分 类 号：S858.74[农业科学—临床兽医学]