石首麋鹿致病性大肠埃希菌的分离鉴定与药敏试验  

Isolation,Identification and Drug Sensitivity Test of Pathogenic Escherichia coli fromElaphurus davidianus in Shishou

作　　者：郭定宗[1] 邹苗[1] 万春云[1] 张韦[1] 郭锐[2] 杨世锦[1] 

机构地区：[1]华中农业大学,湖北武汉430070 [2]湖北省农业科学院,湖北武汉430064

出　　处：《动物医学进展》2016年第3期52-58,共7页Progress In Veterinary Medicine

基　　金：鄂环办(2011)282项目;长江航道整治生态补偿项目

摘　　要：湖北石首麋鹿国家级自然保护区是我国三大麋鹿保护基地之一,自然放养状态的麋鹿易受到大肠埃希菌侵袭并导致死亡。为了弄清石首麋鹿保护区内麋鹿大肠埃希菌感染和流行现状,以及致病性和主要毒力因子,通过药敏试验、生化鉴定、16SrDNA PCR、小鼠毒力试验等方法从病料中分离到15株致病性大肠埃希菌。药敏试验结果显示,分离到的15株大肠埃希菌对庆大霉素、卡那霉素、头孢唑啉的敏感度均达到了100%;其次是对氯霉素、新霉素以及链霉素敏感度均超过60%;耐药性方面,对克林霉素和磺胺嘧啶的耐药度分别为80%和60%。毒力因子PCR结果显示,主要毒力因子为ompA(100.00%)、traTa(80.00%)、iss(80.00%)、iucD(60.00%)、irp2(53.33%)、sta(33.33%)、cva/cvi(26.67%)、vat(20.00%)。National Nature Reserve of Elaphurus davidianus in Shishou is one of the three protection bases in our country,and Elaphurus davidianus in the natural pasture are susceptible to E.coli,which may lead to death.In order to ascertain the current status of E.coli infection and epidemic and find out its pathogenicity and major virulence factors in Elaphurus davidianus in Shishou,we isolated 15 strains of pathogenic E.coli from the samples by the drug sensitivity tests,the biochemical identifications,16 SrDNA PCR and the virulence experiments in mice.The rusult of the drug sensitivity tests showed that 15 strains of E.coli were most susceptible to gentamicin,kanamycin and cefazolin and the sensitivity rate were 100%;and they were all susceptible to chloramphenicol,neomycin and streptomycin,and the sensitivity rate were more than60%;they had a strong drug resistance to clindamycin and sulfadiazine,and the resistant rates were 80%and 60% respectively.The virulence detection results showed that the main virulence factors were ompA（100%）,traTa（80%）,ISS（80%）,iucD（60%）,irp2（53.33%）,sta（33.33%）,cva/cvi（26.67%）and VAT（20%）.

关 键 词：致病性大肠埃希菌 麋鹿 分离鉴定 药敏试验 毒力因子 

分 类 号：S852.612[农业科学—基础兽医学] S858.9[农业科学—兽医学]