乙酰半胱氨酸介导的果蝇蛋白质表达谱的变化  

Effects of N-acetyl Cysteine on Proteomics in Drosophila melanogaster

作　　者：杨帆[1] 江晓勇[1] 冯运[2] 冯杉[2] 邓海腾[1] 

机构地区：[1]清华大学生命科学学院,教育部生物信息学重点实验室,北京100084 [2]清华大学生命科学学院,清华北大生命科学联合中心,北京100084

出　　处：《基因组学与应用生物学》2016年第2期274-280,共7页Genomics and Applied Biology

基　　金：清华大学大学生研究训练计划(1522S0286)资助

摘　　要：N-乙酰半胱氨酸(N-acetyl cysteine,NAC)是常用的抗氧化剂,并被用于慢性阻塞性肺疾病的治疗。本研究通过用富含NAC和不含NAC的营养物培养果蝇,发现NAC可以有效提高果蝇体内谷胱甘肽的(glutathione,GSH)含量,并且提高NADH和NAD+的比例。应用定量蛋白质组学,我们鉴定到了239种蛋白在NAC处理过的果蝇和对照组果蝇中有差异表达;其中227种蛋白的表达上调,12种蛋白的表达下调。上调蛋白主要包括抗氧化酶体系以及GSH合成和利用相关的蛋白。本研究还应用代谢组学方法分析了糖酵解及三羧酸循环通路上的代谢分子,发现NAC可以降低生物体的基础代谢水平。本项研究系统分析了NAC对果蝇的代谢水平、氧化还原稳态以及抗氧化酶体系的影响,阐明NAC在生物体抵抗氧化压力和抗衰老方面的作用。N-acetyl cysteine （NAC） is a widely used antioxidant in research and clinical therapy. In the present study, Drosophila were feed with NAC, and analyzed. We found that NAC-treatment effectively increased the cellular glutathione （GSH） content as well as the NADH/NAD＋ ratio in fruit flies. Using quantitative proteomic technology, we identified 239 differentially expressed proteins between NAC-treated and the control fruit flies, in which 227 proteins were upregulated and 12 proteins were downregulated in NAC-treated fruit flies. Our data suggest that the increase ofNADH/NAD＋ ratio is associated with the upregulation of antioxidant proteins. Based on metabolomic analysis, we show that an amount of metabolic products of glycolysis and citric acid cycle decrease in NAC-treated fruit flies. Our result show NAC treatment upregulates antioxidant enzymes, maintains redox homeostasis and decreases metabolic process, suggesting that NAC has positive effect on cellular resistance to oxidative stress and slowing down aging.

关 键 词：N-乙酰半胱氨酸 黑腹果蝇 定量蛋白质组学 代谢组学 

分 类 号：Q966[生物学—昆虫学]