楸子脱水素基因的克隆及表达分析  

Analysis of Cloning and Expression of Dehydrin Gene in Malus prunifolia

作　　者：高帆[1] 梁东[1] 夏惠[1] 唐月明[1] 徐颖欢 侯帅[1] 

机构地区：[1]四川农业大学果蔬研究所,成都611130

出　　处：《基因组学与应用生物学》2016年第2期436-441,共6页Genomics and Applied Biology

基　　金：国家自然科学基金青年基金项目(31300316)资助

摘　　要：脱水素(dehydrin)在植物抵御非生物逆境胁迫方面具有重要作用。本研究以楸子(Malus prunifolia)为材料,采用改良CTAB法提取RNA,根据苹果的脱水素基因序列设计特异引物,利用反转录聚合酶链式反应(RT-PCR)技术扩增,获得一条楸子脱水素cDNA全长序列。该基因全长874 bp,序列中含有一个720 bp的开放阅读框,编码233个氨基酸,为YK3型脱水素,命名为MpDHN8。氨基酸同源性分析表明,该基因编码的氨基酸序列与苹果(Malus domestica)脱水素基因(AFG33216.1)的同源性高达97%。荧光定量PCR结果表明,在干旱和盐胁迫下,MpDHN8的表达量显著提高,说明该基因作为正向调控因子参与调控楸子的逆境胁迫。Dehydrin plays an important role in against various abiotic stresses in plants. In this paper, a novel total dehydrin cDNA was obtained from Malus prunifolia by using modified CTAB method to extract total RNA, and specific primers designed according to dehydrin sequence of Malus prunifolia, which got amplification by using RT-PCR. This gene that belonged to YK3 type dehydrin was named MpDHN8, with full length of 874 bp containing a 233 bp long ORF and encoding 233 amino acids. Homology analysis of the deduced amino acids with that of other plants indicated that the dehydrin of Malus prunifolia had a similarity of 97% with apple （Malus domestic a） dehydrin 6 （AFG33216.1）. Real-time PCR showed that expression amount of MpDHN8 were signifi- cantly promoted under drought and salt stress, which indicated that this gene plays a role in regulating Malus prunifolia under stresses as a positive regulatory.

关 键 词：楸子 脱水素 基因克隆 基因表达 

分 类 号：S661.1[农业科学—果树学]