疏肝健脾方含药血清对酒精性损伤HepG2细胞脂肪变性的保护作用  

Effect of Shuganjianpi decoction in the prevention of alcohol-induced steatosis in HepG2 cells

作　　者：郭灿[1] 曾莉[1] 

机构地区：[1]成都市第五人民医院中药房,611130

出　　处：《实用肝脏病杂志》2016年第2期160-163,共4页Journal of Practical Hepatology

摘　　要：目的探讨疏肝健脾方含药血清对酒精性损伤HepG2细胞脂肪变性的保护作用。方法采用酒精诱导HepG2细胞损伤模型,设立正常对照组、模型组、含药血清组(疏肝健脾方含药血清)和正常血清组,观察HepG2细胞形态以及生长情况;采用MTT法检测细胞活力,采用油红O染色对存活HepG2细胞脂肪变程度进行观察和量化比较,采用免疫印迹法检测HepG2细胞过氧化物酶体增殖物激活受体(PPAR)γ蛋白表达水平。结果含药血清组细胞形态与正常对照组HepG2细胞形态相似,细胞形态完整,贴壁生长状态良好;含药血清组和正常对照组HepG2细胞脂肪变程度十分接近,脂肪变程度较轻;油红O染色显示,异丙醇溶液脱色后吸光度测定值在模型组为(0.942±0.051),正常血清组为(0.928±0.049),均显著高于含药血清组(0.619±0.043)和正常对照组【(0.621±0.050,P<0.05】;MTT吸光度值在正常对照组为(1.021±0.071),在含药血清组为(0.962±0.066),也显著高于模型组(0.561±0.035)和正常血清组【(0.572±0.041),P<0.05】;正常对照组和含药血清组HepG2细胞PPARγ蛋白表达水平显著低于模型组和正常血清组(P<0.05)。结论疏肝健脾方含药血清对酒精性损伤HepG2细胞脂肪变性有保护作用。Objective To explore the effect of Shuganjianpi decoction in the prevention of alcohol-induced steatosis in HepG2 cells in vitro. Methods Cellular injury of HepG2 cells were induced by alcohol treatment, and HepG2 cells were divided into four groups, e.g. control group（group A）, alcohol-treated group（group B）, serum containing Shuganjianpi decoction treated group（group C） and normal serum treated group（group D）. Cellular morphology and growth were observed. Cell viability was tested by MTT assay. The degree of steatosis in viable cells was evaluated by Oil red O staining, and the expression of peroxisome proliferator-activated receptor-γ（PPAR-γ） was detected by Western blotting assay. Results The cells in group A and group C had similar morphological manifestations and good performance of adherence, and similar mild extent of steatosis;Oil red O staining revealed that after the decolorization by isopropyl alcohol solution, absorbance value in group B（0.942 ± 0.051） and group D（0.928±0.049） were significantly higher than those in group C（0.619±0.043） or in group A [（0.621 ±0.050）, P〈0.05]; MTT assay showed that the absorbance value in group A（1.021 ±0.071） and group C（0.962 ±0.066） were significantly higher than in group B（0.561 ± 0.035） and group D [（0.572 ±0.041）,P〈0.05]; The expression of PPARγ protein decreased in group A and group C than in group B and group D did（P〈0.05）. Conclusions The effect of Shuganjianpi decoction is effective in the prevention of alcohol-induced steatosis in HepG2 cells.

关 键 词：酒精性脂肪肝 HEPG2细胞 疏肝健脾方 脂肪代谢 

分 类 号：R285[医药卫生—中药学]