鹦鹉热衣原体的B598_0590基因编码包涵体膜蛋白  

Chlamydia psittaci B598_0590 Gene Encodes an Inclusion Membrane Protein

作　　者：李鹏[1,2] 何君[1] 朱虹[1] 李岩伟[1] 于永慧[1] 端青[1] 宋立华[1] 

机构地区：[1]病原微生物生物安全国家重点实验室,军事医学科学院微生物流行病研究所,北京100071 [2]解放军第264医院,山西太原030001

出　　处：《生物技术通讯》2016年第1期17-20,共4页Letters in Biotechnology

基　　金：“十二五”农村领域国家科技计划(2012AA101302)

摘　　要：目的:鹦鹉热衣原体的B598_0590基因与沙眼衣原体的毒力基因CT135同源,本研究旨在分析该基因的表达和定位。方法:生物信息学方法分析B598_0590基因的进化地位,比较B598_0590蛋白和沙眼衣原体毒力蛋白CT135的氨基酸疏水特征;重组表达、纯化鹦鹉热衣原体的B598_0590蛋白,免疫小鼠制备抗血清;共聚焦免疫荧光观察鹦鹉衣原体在正常培养条件和使用Lpx C抑制剂时B598_0590基因的表达和定位。结果:衣原体属内12个种的基因组均含有CT135同源基因,它们编码的蛋白质有相似的疏水特征;B598_0590与CT135的氨基酸同源性为21%;B598_0590的免疫荧光染色特征与包涵体膜蛋白Inc A相似,浓染包涵体膜;Lpx C抑制剂可抑制网状体的分裂、包涵体的生长及网状体向原体转化,包涵体膜蛋白的染色呈现典型的空泡结构。结论:Lpx C抑制剂可用于鉴定未知的鹦鹉热衣原体包涵体膜蛋白;鹦鹉热衣原体的B598_0590基因编码此前尚未鉴定的包涵体膜蛋白。Objective： To analyze the expression and localization of Chlamydia psittaci B598_0590 gene, a para- log of C.trachomatis virulence gene CT135. Methods： Phylogenetic analysis of B598_0590 within Chlamydia ge- nus, and hydrophobic comparison of B598_0590 and CT135 protein were performed. Mouse sera against recombi- nant B598_0590 of C.psittaci were prepared. The expression and localization of B598_0590 under normal culture conditions and under LpxC inhibitor treatments, were observed by using confocal microscopy. Results： All the Chlamydia species have a CT135 paralog, and have similar hydrophobic characteristics. Proteins deduced from B598_0590 and CT135 share 21% amino acid homology. The immunofluorescence characteristics of B598_0590 and inclusion membrane protein IncA were similar. LpxC inhibitor can inhibit normal fissions of reticulate body, in- clusion growth, and conversion from reticulate body to elementary body. Immunofluorescence of inclusion membrane proteins under LpxC inhibitor treatments shows characteristic vacuole structures. Conclusion： LpxC inhibitors can be used for identifying C.psittaci inclusion membrane proteins. The C.psittaci B598_0590 gene encodes a previous- ly uncharacterized inclusion membrane protein.

关 键 词：鹦鹉热衣原体 包涵体膜蛋白 蛋白定位 CT135 B598_0590 

分 类 号：R374.2[医药卫生—病原生物学] Q811.4[医药卫生—基础医学]