促吞噬肽衍生物TP对树突状细胞功能的影响  

Tuftsin-Derivated TP Affects Dendritic Cells Function

作　　者：安映红[1] 宣自学 韩苏[3] 杨德宣[2] 徐城望 袁守军[2] 

机构地区：[1]空军总医院临床检验中心生化室,北京100142 [2]军事医学科学院放射与辐射医学研究所,北京100850 [3]南开大学药学院,天津300071

出　　处：《生物技术通讯》2016年第1期66-68,共3页Letters in Biotechnology

基　　金：重大新药创制国家科技重大专项综合新药研究开发技术大平台项目(2012ZX09301003-001);国家自然科学基金(30973564)

摘　　要：目的:探讨促吞噬肽衍生物TP对树突状细胞(DC)功能的影响。方法:分离、提纯小鼠骨髓来源DC,培养至第3 d弃上清,去除悬浮细胞,加入新鲜培养液,补充细胞因子,之后隔天半量换液,至第7 d获得DC;分别用TP和LPS刺激DC,Wright-Gimsa染色,用倒置显微镜观察细胞生长情况;流式细胞分析细胞表面分子CD80、CD83、CD86及CD11c的表达,鉴定细胞成熟度;q RT-PCR分析TP对DC分泌白细胞介素12b(IL-12b)的影响。结果:光镜及Wright-Gimsa染色的细胞形态学结果都显示TP可促进DC增殖及成熟,流式分析证明了这一结果,同时TP还促进DC大量分泌IL-12b。结论:TP不仅能促进DC增殖及成熟,还能影响DC的IL-12b表达;DC有可能是TP发挥抗癌作用的靶细胞之一。Objective： Investigating the effect of tuftsin-derivated TP on dendritic cells（DC） function. Methods： Murine bone marrow-derived DC were isolated and purified. When cultured for the third days, the suspended cells were removed and fresh culture mediums were added with supplemental cytokines. Half of the fluids were re- placed every other day. Then DC were received on the seventh day. TP cells were stimulated by DC and Wright- Gimsa, and CD83, CD86, TP and CDllc were detected by LPS and qRT-PCR. Results： Both light microscopy and Wright-Gimsa staining showed that TP could promote the proliferation and maturation of DC. TP significantly promoted DC to secret IL-12b, too. Conclusion： TP can not only promote the proliferation and maturation of DC, but also affect the IL-12b expression of DC. DC may be one of the target cells for TP to play critical role in an- ti-cancer.

关 键 词：促吞噬肽 TP 树突状细胞 细胞因子 白细胞介素12b 

分 类 号：R965.1[医药卫生—药理学]