STAT3对PTEN缺失的HGC27细胞增殖的影响  

Effect of STAT3 on the Proliferation of PTEN- Deficient HGC27 Cells

作　　者：田秉昌 卫勃[2] 乔振宇[3] 吕晓业 黄芳[4] 王芃[4] 李山虎[4] 周建光[4] 王健[4] 刘文忠[1] 

机构地区：[1]内蒙古医科大学基础医学院,内蒙古呼和浩特010059 [2]解放军总医院普外科,北京100853 [3]贵阳医学院附属医院,贵州贵阳550001 [4]军事医学科学院生物工程研究所,北京100850

出　　处：《生物技术通讯》2016年第1期76-78,共3页Letters in Biotechnology

基　　金：国家自然科学基金(81372740,81372770,81470138)

摘　　要：目的:初步探索STAT3与HGC27胃癌细胞增殖的关系。方法:用T4 DNA连接酶将外源片段与酶切后的p LKO.1载体连接,将构建的重组质粒转染293T细胞,48 h后收集上清用于感染HGC27细胞,Western印迹检测蛋白的表达,生长实验检测其对肿瘤细胞增殖的影响。结果:基因测序和双酶切鉴定表明敲低STAT3质粒构建成功;慢病毒质粒有效抑制HGC27细胞中STAT3蛋白水平,抑制STAT3对HGC27细胞的增殖有明显抑制作用。结论:在PTEN缺失的HGC27胃癌细胞中STAT3促进肿瘤增殖,但STAT3并不是在所有PTEN缺失的肿瘤细胞中都发挥抑制肿瘤形成的作用。Objective： To explore the relationship between STAT3 and tumor cell proliferation in HGC27 cells. Methods： Exogenous fragment was cloned into the pLKO.1 vector, the recombinant plasmid was transfected into 293T cells lines and produced lentiviral particles. HGC27 was infected and examined by Western blotting. In addi- tion, after infecting HGC27 cells, the proliferation of cells was examined using CCK8. Results： Gene sequencing and double enzyme digestion showed that the construction of the lentivious plasmid was successful. The construct- ed plasmid can inhibit the STAT3 protein level of HGC27 cells, the proliferation of HGC27 cells was inhibited af- ter STAT3 expression was inhibited. Conclusion： In PTEN-deficient HGC27 gastric cancer cells, STAT3 promotes tumor growth. STAT3 don＇t play an inhibiting role in the all PTEN-deficient tumor cell, which the phenomenon can provide some guidance for clinical chemotherapy.

关 键 词：STAT3 PTEN 胃癌 HGC27细胞 

分 类 号：Q78[生物学—分子生物学] Q25