Xpert MTB/RIF系统在盆腔结核诊断中的应用价值分析  

Application of Xpert MTB / RIF Assay in the Diagnosis of Pelvic Tuberculosis

作　　者：柳晓金[1] 赵辉[2] 马建军[3] 许怡[1] 高会霞[1] 

机构地区：[1]石家庄市第五医院检验科,河北石家庄050021 [2]石家庄市第五医院功能科,河北石家庄050021 [3]石家庄市第五医院影像科,河北石家庄050021

出　　处：《标记免疫分析与临床》2016年第2期147-149,共3页Labeled Immunoassays and Clinical Medicine

基　　金：河北省医学科学研究重点课题计划(20150894)

摘　　要：目的探讨Xpert MTB/RIF系统检测盆腔积液中结核分枝杆菌对于盆腔结核的诊断价值。方法采集30例临床确诊为盆腔结核患者及30例盆腔恶性肿瘤患者(对照组)的盆腔积液,对积液同时采用离心涂片法、荧光定量PCR法和Xpert MTB/RIF系统进行结核分枝杆菌检测,比较三种方法的敏感性及特异性。结果离心涂片法、荧光定量PCR法和Xpert MTB/RIF系统检测结核分枝杆菌的敏感性分别为6.67%(2/30)、53.33%(16/30)和73.33%(22/30),三种方法间差异均有统计学意义(P均<0.05)。三种方法特异度分别为100.0%,96.7%,100.0%,差异无统计学意义。结论采用Xpert MTB/RIF系统对盆腔积液进行结核分枝杆菌检测敏感性高,特异度好,具有较高的临床应用价值。Objective To investigate the application of Xpert MTB / RIF assay in the diagnosis of pelvic tuberculosis. Methods 30 patients clinically diagnosed with pelvic tuberculosis and 30 patients with pelvic tumor were enrolled. The pelvic fluid was obtained by pelvic centesis and detected by centrifuged smears,real-time PCR and Xpert MTB / RIF assay for Mycobacterium tuberculosis（ MTB）. Then sensitivities and specificities of results were compared. Results The sensitivity of Xpert MTB / RIF assay was 73. 33%,higher than that of centrifuged smears（ 6. 67%） and real-time PCR（ 53. 33%）. The results had statistical significance（ χ2=18. 05,P 〈 0. 001 and χ2= 4. 17,P 〈 0. 05）. The specificities of centrifuged smears,real-time PCR and Xpert MTB / RIF assay were 100. 0%,96. 7%,100. 0%. Conclusion Xpert MTB / RIF assay is a specific and sensitive approach in detecting MTB in pelvic fluid.

关 键 词：结核 盆腔积液 Xpert MTB/RIF 

分 类 号：R529.0[医药卫生—内科学] R440[医药卫生—临床医学]