子痫前期血清通过Caveolin-1增加肾小球内皮细胞通透性  

The high expression of caveolin-1 causes the hyperpermeability of human glomerular endothelial cells in preeclampsia patients

作　　者：徐钦洋 杜菲[1] 蒋荣珍[1] 

机构地区：[1]上海交通大学附属第六人民医院产科重症监护中心妇产科研究所,上海200233

出　　处：《现代妇产科进展》2016年第1期10-13,18,共5页Progress in Obstetrics and Gynecology

基　　金：国家自然科学基金资助项目(No:81570444);国家科技部"十二五"支撑计划项目(No:2014BAI05B05);上海交通大学医学院健康管理研究基金(LY201501)

摘　　要：目的:探讨子痫前期血清对肾小球内皮细胞(HRGEC)通透性的影响以及caveolin-1表达在子痫前期血清影响HRGEC通透性过程中的作用。方法:采用原代培养的肾小球内皮细胞,实验分组:正常妊娠血清培养组(A组),子痫前期血清培养组(B组),子痫前期血清+caveolae蛋白的抑制剂甲基-β-环糊精(MBCD)处理组(C组)。免疫荧光及免疫印迹监测HRGEC caveolin-1蛋白表达;细胞接种transwell小室,Evens-blue检测HRGEC单层细胞对大分子蛋白通透性改变;Western blot法检测caveolin-1表达。结果:子痫前期血清明显促进单层HRGEC对大分子蛋白的通透性与caveolin-1表达(P<0.05),MBCD能拮抗子痫前期血清促单层HRGEC对大分子蛋白的通透性与caveolin-1表达的作用。结论:子痫前期患者血清通过增加caveolin-1蛋白表达促进HRGEC单层细胞对大分子蛋白的通透性,caveolin-1表达异常可能在子痫前期患者蛋白尿形成过程中起重要作用。Objective： To explore the serum impacts on the permeability of human renal glomerular endothelial cells（ HRGEC） in preeclampsia patients and the function of expressed caveolin-1 on the permeability of HRGEC. Method： To culture the primary HRGEC and observe the position of Caveolin-1 in HRGEC by immunofluorescence. Then culture HRGEC in a device called ＂transwell＂. Three groups were divided： Group A was treated with the serum of normal pregnant when the cells overgrew the whole upper chamber. Group B was treated with the preeclampsia patients serum. Group C was treated with the same preeclampsia serum as the second group and also one caveolae inhibitor- Methyl-beta- cyclodextrinthe（ MBCD） in a suitable concentration. And then test the Evans-bule conjuncted BSA in the lower chamber to reflect the dynamic permeability of HRGEC. Western blot was used to detect the quantity of caveolin-1 expressed by HRGEC after the cells were stimulated by serums of each group for 24 hours. Result： the permeability of HRGEC was significantly enhanced when they were dealt with preeclampsia serum and the inhibitor could reverse them partly（ P 〈0. 05）. The expression of caveolin-1 was highly elevated（ P 〈0. 05）,it was reduced when treated with MBCD（ P 〈0. 05）. Conclusion： High expressed caveolin-1in preeclampsia patients can lead to hyperpermeability of HRGEC,which may play an important role in proteinuria formation in preeclampsia patients.

关 键 词：子痫前期 肾小球内皮细胞 CAVEOLIN-1 屏障障碍 蛋白尿 

分 类 号：R714.2[医药卫生—妇产科学]