河南省HIV携带者刚地弓形虫感染情况  

Toxoplasma gondii Infection in HIV-infected Individuals in Henan Province

作　　者：白晨倩 王东[1] 姚志军[1] 王帅[1] 张露文[1] 刁风金 任笑盈 何婵婵 王兆红[1] 马金超[1] 王苏康 彭帮淦 胡翠[1] 安戈[2] 刘世国[1] 

机构地区：[1]新乡医学院基础医学院人体寄生虫学教研室,新乡453000 [2]郑州市疾病预防控制中心,郑州450000

出　　处：《中国寄生虫学与寄生虫病杂志》2016年第1期84-86,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：河南省教育厅科学技术研究重点项目(No.13A310845);新乡医学院博士科研启动基金(No.XYBKYZZ201504)~~

摘　　要：收集河南省1 038份HIV携带者外周血样,提取其基因组DNA后,利用巢式PCR技术扩增弓形虫529 bp重复序列基因,凝胶电泳检测目的片段。1 038份HIV携带者全血血样中（男762份,女276份）,弓形虫PCR阳性血样66份,弓形虫PCR阳性率为6.4%（66/1 038）。男性和女性的弓形虫PCR阳性率分别为6.3%（48/762）和6.5%（18/276）。已婚508份,未婚、离异或丧偶530份,其弓形虫PCR阳性率分别为4.9%（25/508）和7.7%（41/530）（χ^2=3.451,P〉0.05）。高中及以上学历、高中以下学历人群的弓形虫PCR阳性率分别为6.9%（34/489）和5.8%（32/549）（χ^2=0.545,P〉0.05）。20~40岁组人群的弓形虫PCR阳性率最高,为7.6%（31/410）。有性病史、无性病史和病史不详者的弓形虫PCR阳性率分别为8.0%（9/113）、6.5%（50/773）和4.6%（7/152）（χ^2=0.355,P〉0.05）。Genomic DNA was extracted from 1 038 peripheral blood samples from HIV-infected individuals in Henan Province. One-step single-tube nested PCR was performed to amplify the 529 bp repeating sequences of Toxoplasma gondii. Of the 1 038 samples（762 from males and 276 from females）, 66 showed positive PCR results,with a positive rate of 6.4%. The PCR positive rate in males and females was 6.3%（48/762） and 6.5%（18/276）respectively. The PCR positive rate in the married HIV individuals was 4.9%（25/508）, and that in unmarried,divorced and widowed HIV individuals was 7.7%（41/530）（χ^2= 3.451, P〉 0.05）. The PCR positive rate in HIV individuals with a high-school educational level or above was 6.9%（34/489）, and that in those below the high-school level was 5.8%（32/549）（χ^2= 0.545, P 〉0.05）. The highest infection rate was in the age group of 20-40years（7.6%, 31/410）. In addition, the Toxoplasma infection rate in those with and without a history of venereal diseases, and those with an unknown history was 8.0%（9/113）, 6.5%（50/773） and 4.6%（7/152） respectively（χ^2=0.355, P 〉0.05）.

关 键 词：艾滋病病毒 刚地弓形虫 巢式PCR 河南 

分 类 号：R382.5[医药卫生—医学寄生虫学]