分离自蛇体的侵入内阿米巴滋养体的培养与应用  

Culture and Application of Entamoeba invadens Trophozoites from Snake

作　　者：齐莉莉[1] 贾娴娴[1] 杨晓红[1] 赵博[2] 王贺[1] 

机构地区：[1]河北医科大学基础医学院,石家庄050017 [2]河北医科大学教务处,石家庄050017

出　　处：《中国寄生虫学与寄生虫病杂志》2016年第1期87-88,共2页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：将分离自蛇体的侵入内阿米巴(Entamoeba invadens)滋养体接种于营养琼脂血清盐水培养基,22℃恒温培养,每周转种1次。转种后的培养液内含大量滋养体,制成玻片标本,显微镜下观察虫体形态。侵入内阿米巴滋养体的形态变化特点、运动方式、生殖周期和侵袭力等与感染人的溶组织内阿米巴滋养体基本相同。以侵入内阿米巴滋养体代替溶组织内阿米巴滋养体,学生能观察到活体阿米巴滋养体。The trophozoites of Entamoeba invadens from snake were seeded in liquid medium, incubated at22 ℃ under constant temperature, and transferred weekly. The liquid medium which contained a large number of trophozoites was used for preparation of samples for microscopic observation. The cultured trophozoites of snake E. invadens displayed similar morphological changes, movement patterns, reproductive cycle and invasiveness with human E. histolytica. Therefore, the snake E. invadens trophozoites can be used as an alternative to the human E. histolytica trophozoites to facilitate students ＇ observation of living amoeba trophozoites.

关 键 词：侵入内阿米巴 滋养体 培养 

分 类 号：R382.1[医药卫生—医学寄生虫学]