芽孢杆菌1205和烟草疫霉处理后对烟苗防御酶的测定  

Measurement of Defense Enzyme in Tobacco Processed with Bacillus subtilis 1205 and Phytophthora parasitica Gzufp-9

作　　者：施渺筱[1] 郭博恺 万科[2] 丛铭[3] 张传萍[3] 李祝[3] 

机构地区：[1]安顺学院农学院,贵州安顺561000 [2]贵州大学药学院,贵阳550025 [3]贵州大学生命科学学院真菌资源研究所,贵阳550025

出　　处：《湖北农业科学》2016年第2期362-364,共3页Hubei Agricultural Sciences

基　　金：贵州省科学技术厅;安顺市人民政府;安顺学院联合科技基金项目[黔科合J字LKA(2012)06号];毕节市烟草公司项目(2011.03)

摘　　要：分别将烟苗接种芽孢杆菌(Bacillus subtilis)1205和烟草黑胫病菌病原菌烟草疫霉(Phytophthora parasitica Gzufp-9)在接种后1、3、5、7、9、11 d测定烟苗过氧化物酶(POD)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)3种与植物抗病密切相关的酶活性变化。结果表明,接种芽孢杆菌1 205后,PAL、PPO活性在5 d时达到最高值,明显高于对照和接种烟草疫霉,POD活性在接种1 d时即达到最高值。而接种烟草疫霉后PAL、PPO活性均在3 d时达到最高值,POD活性在接种1 d时达到最高值。After the respective inoculation of Bacillus subtilis 1205 and Phytophthora parasitica Gzufp-9 pathogenic bacteria into tobacco seedling, the change in activity of disease-resistant enzymes such as POD, PPO, PAL were tested and recorded on 1^st, 3^rd, 5^th, 7^th, 9^th, and 11 thday. The results showed that after the inoculation of B. subtilis 1 205, the activity of PAL and PPO reached its peak on the 5thday, apparently higher than the control group and the ones with P. parasitica; POD reached its peak on the 1st day. After the inoculation of P. parasitica, the activity of PAL and PPO reached its peak on the3 rd day, POD reached its peak on the 1st day.

关 键 词：烟草疫霉(Phytophthora parasitica Gzufp-9) 芽孢杆菌(Bacillus subtilis) 抗性相关酶 

分 类 号：S432.44[农业科学—植物病理学]