响应面优化超声波-酶法提取丹参中丹酚酸B的工艺研究  

Optimization for Ultrasonic-enzymatic Extraction Process of Salvianolic Acid B from Salvia miltiorrhiza Bunge by Response Surface Methodology

作　　者：宁娜[1] 韩建军[1] 郁建生[1] 邹宗尧 牟少霞 

机构地区：[1]铜仁职业技术学院药学院,贵州铜仁554300 [2]西南大学药学院,重庆400715

出　　处：《湖北农业科学》2016年第2期444-448,共5页Hubei Agricultural Sciences

基　　金：国家科研平台建设项目[发改高技(2013)2064号];贵州省高层次人才科研条件特助经费项目[黔人领发(2012)3号];贵州省铜仁市科技局科研计划项目[铜仁市研(2012)62号]

摘　　要：利用响应面分析法对丹参(Salvia miltiorrhiza Bunge)中丹酚酸B的超声波协同酶法提取工艺进行优化。在单因素试验基础上,选取酶解时间、酶解温度、纤维素酶用量和酶解p H进行4因素3水平的Box-Behnken中心组合研究。通过响应面分析法对提取条件进行了优化,确定超声波协同酶法提取丹参中丹酚酸B的最佳工艺为酶解时间54 min,酶解温度49℃,纤维素酶用量2.70 mg/g、酶解p H 4.4。在该条件下,丹参中丹酚酸B的提取量为4.83 mg/g。Based on response surface methodology（RSM）,the conditions for ultrasonic-enzymatic synergistic extraction of salvianolic acid B from Salvia miltiorrhiza Bunge was optimized. Experimental factors and their levels were determined by singe l-factor tests. Subsequently, the center combination experimental design of Box-Behnken was used to investigate the effects of enzymolysis time, enzymolysis temperature, cellulose dosage and p H value on the extraction rate of salvianolic acid B. The optimum extraction conditions were determined by RSM. The results showed that the optimum extraction conditions were as follows： enzymolysis time was 54 min, enzymolysis temperature was 49 ℃,cellulose dosage was 2.7 mg / g,p H value was 4.4.Under these conditions, the extraction yield of salvianolic acid B was 4.83 mg / g.

关 键 词：丹参(Salvia miltiorrhiza Bunge) 丹酚酸B 纤维素酶 超声法 

分 类 号：S567.5[农业科学—中草药栽培] R284.2[农业科学—作物学]