纳米Fe_3O_4-TiO_2靶向光动力疗法对肝癌细胞的杀伤效应（英文）  

Photocatalytic Killing Effect of Fe_3O_4-TiO_2 Nanoparticles on Hepatoma Carcinoma Cells for Targeting Photodynamic Therapy

作　　者：王玲[1] 文雯[1] 李莎[1] 付正卿 仝月菊[1] 王娟[1] 韩冬[1] 张爱平[1] 

机构地区：[1]山西医科大学药学院,太原030001

出　　处：《无机化学学报》2016年第3期393-404,共12页Chinese Journal of Inorganic Chemistry

基　　金：山西省自然科学基金(No.2015011012)资助项目

摘　　要：光动力疗法(PDT)作为一种迅速发展的传统替代疗法,在抗癌治疗中显示出巨大的潜力。为增强靶向性和提高光催化杀伤效率,本研究设计了一种新型光敏剂Fe_3O_4-Ti O_2磁性纳米粒。在不同外磁场下,考察其在可见光和紫外光激发下对肝癌细胞的杀伤效应。同时利用流式细胞术检测纳米Fe_3O_4-Ti O_2对肝癌细胞凋亡率、细胞周期和线粒体膜电位的影响。根据纳米Fe_3O_4-Ti O_2和肝癌细胞的作用方式探讨其抗癌机制。结果表明,可见光激发纳米Fe_3O_4-Ti O_2可以杀伤癌细胞,且其杀伤效率与紫外光激发下无明显差别。此外,Fe_3O_4-Ti O_2比Ti O_2具有更高的细胞摄取率,从而使其具有更高的选择性和光催化杀伤效率。其作用机制是光催化纳米Fe_3O_4-Ti O_2产生活性氧ROS抑制癌细胞,然后通过阻滞细胞周期G_0/G_1期,降低线粒体膜电位,线粒体去极化,最终诱导细胞凋亡。Photodynamic therapy（PDT）, as a fast developing alternative of traditional therapeutics, is showing great promise in antitumor treatment. Herein, novel photosensitizer（PS） Fe3O4-Ti O2 magnetic nanoparticles（NPs）were strategically designed as targeting drug with higher photocatalytic killing efficiency. The photokilling effect of Fe3O4-Ti O2 NPs on hepatoma carcinoma cells（Hep G2） in different external magnetic fields was investigated under ultraviolet light and visible light irradiation. Meanwhile, changes of cell cycle, apoptosis rate and mitochondrial membrane potential（MMP） of Hep G2 cells were detected by flow cytometry（FCM）. The results indicated that Fe3O4-Ti O2 NPs have comparable photokilling efficiency to Hep G2 cells under both visible and ultraviolet light. Moreover, Fe3O4-Ti O2 NPs have higher cell uptake efficiency than pure Ti O2, which made it possible to have higher selectivity and photocatalytic killing efficiency. The mechanism was that photocatalytic Fe3O4-Ti O2 NPs inhibit cancer cells by yielding reactive oxygen species（ROS）. Then, Fe3O4-Ti O2 induced apoptosis, mediated by arresting cell cycle in G0/G1 phase, reducing mitochondrial membrane potential, and mitochondrial depolarization.

关 键 词：光动力疗法 Fe3O4-TiO2纳米粒 光催化 机制 肝癌细胞 

分 类 号：O649[理学—物理化学] Q291[理学—化学]