刺参苗期附着基更换频率对刺参生长及其养殖系统菌群结构的影响  

Effects of substrate change frequency on growth and variation of the microflora structure in a seedling cultivation system of sea cucumber Apostichopus japonicus

作　　者：王印庚[1] 张文泽[1,2] 廖梅杰[1] 李彬[1] 荣小军[1] 张正[1] 李华[2] 范瑞用 宁鲁光 

机构地区：[1]中国水产科学研究院黄海水产研究所,青岛海洋科学与技术国家实验室,海洋渔业科学与食物产出过程功能实验室,山东青岛266071 [2]大连海洋大学水产与生命学院,辽宁大连116023 [3]青岛瑞滋海珍品发展有限公司,山东青岛266400 [4]东营海跃水产科技有限公司,山东东营257500

出　　处：《中国水产科学》2016年第2期359-369,共11页Journal of Fishery Sciences of China

基　　金：国家自然科学基金资助项目(31202016);国家863计划项目(2012AA10A412-4);山东省农业良种工程重大课题"速生;抗病;耐高温刺参良种选育";山东省自主创新成果转化专项(2013ZHZX2A0801)

摘　　要：为了探究刺参（Apoasichopus japonicus）保苗阶段（7–9月）最佳的附着基更换频率（changing frequency,CF）,本实验在夏季保苗期设置5个附着基更换频率组,即CF10、CF20、CF30、CF40和CF50。采用实验生态学的方法,并结合传统细菌培养法和16S r DNA细菌鉴定技术对上述不同实验组进行检测。结果表明：CF20组刺参整池增重和个体增重幅度最大,CF30次之,CF50组由于死亡率高,整池重量为负增长。CF20组的特定生长率和存活率分别为（5.986±0.135）%/d和（95.231±0.265）/%,且显著高于其他各组（P〈0.05）,CF30次之,而CF_（50）组的特定生长率和存活率最低,且显著低于其他各组（P〈0.05）。养殖用水中4NH＋-N、2NO--N和COD随着附着基更换频率的降低而升高,并在第50天时分别达到0.53 mg/L、0.28 mg/L、0.18 mg/L。各实验组水体中异养细菌和弧菌数量随附着基更换频率变化不明显,而附着基上的异养细菌和弧菌数量随附着基更换频率的降低而升高,CF_（50）组异养细菌总数在第50天时达到1.38×10^5 cfu/cm^2,弧菌数量达到1.5×10^4 cfu/cm^2,皆明显高于其他各组。附着基上优势菌为溶藻弧菌（Vibrio algindyticus）、需钠弧菌（V.natriegens）、马胃葡萄球菌（Staphylococcus equorum）、枯草芽孢杆菌（Bacillus subtilis）、苏云金芽孢杆菌（B.thuringiensis）和副溶血弧菌（V.parahaemolyticus）。其中,溶藻弧菌为刺参条件致病菌,且一直存在于养殖系统中并逐步占据绝对优势。这与CF_（50）组在实验进行到43 d时开始出现化皮,50 d时开始出现死亡现象有一定的关系。同时,附着基长时间未更换,会滋生大量玻璃海鞘、日本毛壶、内刺盘管虫等敌害生物,争夺栖息空间和食物,导致刺参苗种生长减慢。综上,由实验结果显示,在7–9月高温季节每20 d更换一次附着基最佳。考虑到生产成本,附着基更换频率一般为20~30 d为宜。本研究结果为�To determine the best change frequency of substrates in the sea cucumber seedling cultivation system in summer, the relationship between the substrate change frequency and growth of sea cucumbers, and the microflora structure on the substrate, was investigated using an experimental ecology method, a traditional bacterial culture method and 16 S r DNA sequence analysis. Five different substrate change frequency groups（10 d, 20 d, 30 d, 40 d, 50 d） were set and termed CF10, CF20, CF_（30）, CF40 and CF_（50）. Judged by the weight gain of the whole cultivation tank and the main body weight, the group achieved the best growth rate, followed by the CF_（30） group. The CF_（50） group achieved the highest death rate, which caused a decrease in the weight of the whole tank. The specific growth rate and the survival rate of the CF20 group were（5.986±0.135）%/d and（95.231±0.265）%, respectively, which were significantly higher than the other groups（P〈0.05）. The concentration of ammonia nitrogen（4NH＋-N）, nitrite（2NO--N） and chemical oxygen demand（COD） of the pond water increased along with the decrease in the change frequency of substrates, reaching 0.53 mg/L, 0.28 mg/L and 0.18 mg/L on the 50 th day, respectively. In terms of the concentrations of heterotrophic bacteria and vibrios in the pond water and on the substrate, the concentrations of heterotrophic bacteria and vibrios in the pond water did not change significantly with the change frequency of substrates; however, the concentrations of heterotrophic bacteria and vibrios on the substrate increased with decreasing change frequency of substrates, reaching 1.38×10^5 cfu/cm^2 and 1.5×10^4 cfu/cm^2 on the 50 th day in the CF_（50） group, respectively. Bacterial identification showed that the dominant bacteria on the substrates were Vibrio alginolyticus, Vibrio natriegens, Staphylococcus equorum, Bacilllus subtilis, Bacilllus thuringiensis and Vibrio parahaemolyticus, of which V. alginolyticus was the dominant bacteria. V. a

关 键 词：刺参 育苗工艺 附着基 更换频率 生长 菌群结构 弧菌 

分 类 号：S96[农业科学—水产养殖]