中国大豆主栽品种遗传转化效率相关性状的比较研究  

Comparative studies on traits related to Agrobacterium-mediated transformation efficiency of popular soybean cultivars in China

作　　者：陈普[1,2] 陈莉[2] 韩天富[2] 东方阳[1] 侯文胜[2] 

机构地区：[1]河北科技师范学院生命科技学院,河北秦皇岛066004 [2]中国农业科学院作物科学研究所/国家植物转基因技术研究中心/农业部北京大豆生物学重点实验室,北京100081

出　　处：《中国油料作物学报》2016年第1期27-33,共7页Chinese Journal of Oil Crop Sciences

基　　金：转基因生物新品种培育重大专项(2014ZX08010-004);农业部财政部现代农业产业技术体系建设专项(CARS-04);中国农业科学院科技创新工程

摘　　要：受体基因型依赖是限制根癌农杆菌介导的大豆遗传转化效率的重要因素,严重制约优良转基因材料创制和品种选育效率。本实验以适宜我国不同地域种植的13个大豆主栽品种为材料,系统比较了其丛生芽诱导率、芽伸长率和遗传转化效率的差异。结果表明,在根癌农杆菌侵染和筛选剂存在的条件下,供试品种的丛生芽诱导率差异较小,芽伸长率差异较大,最终的遗传转化效率差异更大。在供试的14个大豆品种中,有7个获得转化植株,其中4个转化效率在2%以上。高蛋白品种中黄42的转化效率可达4.1%,是培育转基因大豆的良好受体材料。Agrobacterium tumefaciens- mediated transformation of soybean is highly genotype- dependent,which strongly limits the effective progress for transformation. In this study,13 elite soybean cultivars from different producing region were selected for comparison of their shoot induction,shoot elongation and transformation efficiency. The results showed that there were apparent varietal variation in the shoot induction and significant differences in shoot elongation efficiencies after Agrobacterium infection and glufosinate screening,which lead to large varietals variation of transformation efficiency. Transgenic plants were obtained from 7 out of 14 cultivars,with the transformation efficiency over 2% in 4 cultivars. Zhonghuang 42,a high protein cultivar,possessed the highest transformation efficiency of 4. 1%,which can be used as a receptor material in transgenic soybean breeding in the future.

关 键 词：大豆 根癌农杆菌 丛生芽诱导 芽伸长 转化效率 

分 类 号：S565.103[农业科学—作物学]