花生根际产IAA菌的筛选鉴定及其效应研究  

Isolation and identification of IAA-producing strains from peanut rhizosphere and its promoting effects on peanut growth

作　　者：张东艳[1,2] 刘晔[1] 吴越 王国文[1] 万兵兵[1] 姜瑛[1] 

机构地区：[1]河南农业大学资源与环境学院,河南郑州450002 [2]西南大学资源环境学院,重庆400715 [3]山东省土壤肥料总站,山东济南250100

出　　处：《中国油料作物学报》2016年第1期104-110,共7页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(41401274);“十二五”农村领域国家科技计划课题(2012BAD05B0207)

摘　　要：植物根际产IAA菌能够分泌吲哚乙酸(IAA)促进生长,提高作物产量。为从砂质潮土中的花生根际筛选高产IAA的根际促生菌,本研究首先从长势较好的花生根际筛选出单株菌落,对其分泌IAA能力进行定性和定量分析,并通过16S r DNA基因序列配合生理生化特征鉴定所筛选的高产IAA菌株,然后设置单因素试验,探究适宜菌株生长和发酵的条件,最后通过花生盆栽试验验证其促生能力。结果表明,共筛选出5株产IAA的促生菌菌株,其中菌株HS10的IAA产量最高,被鉴定为特基拉芽孢杆菌。单因素试验表明,HS10最适培养条件是培养温度30℃,时间20 h,初始p H 8,装液量25 m L/250 m L,碳源为果糖,氮源为硝酸钾。盆栽试验表明,接菌花生植株长势明显优于对照,HS10对花生具有良好的促生效应。Some plant rhizosphere bacteria can secrete indoleacetic acid（ IAA） to promote the plant growth and increase crop yield. This research aimed to screen IAA- producing strains from rhizosphere of peanut in sandy soil,and to analyze their promoting effect. 5 IAA- producing strains were isolated from rhizosphere of a healthy peanut plant. Among them,strain HS10 had the highest IAA- production ability and was identified as Bacillus tequilensis by 16 S r RNA gene sequencing combined with its physiological and biochemical test evaluation. HS10 produced maximum IAA at 24 h of incubation with optimum conditions as 30℃,pH8,fluid volume 25 mL /250 mL,fructose and potassium nitrate as carbon source and nitrogen source respectively. Pot experiment demonstrated that HS10 significantly promoted plant growth and root system of peanut compared to the control.

关 键 词：花生 根际产IAA菌 吲哚乙酸 砂质潮土 盆栽试验 特基拉芽孢杆菌 

分 类 号：S154.39[农业科学—土壤学]