内标-标准曲线气相色谱法测定血液中的乙醇含量  

Internal standard-standard curve of gaschromatographic method for the determination of blood alcohol content

作　　者：陈迪勇 宋光林 李荣华 李占彬 

机构地区：[1]贵州省分析测试研究院,贵州贵阳550002

出　　处：《贵州科学》2016年第1期81-84,共4页Guizhou Science

基　　金：国家国际科技合作专项资助(项目编号是2011DFB41640)

摘　　要：目的：建立内标一标准曲线法定量测定血液中的乙醇含量的气相色谱方法，并与SF／ZJD0107001-2010进行比较。方法：用移液管量取0．20mL血样及1．00mL40．0μg／mL叔丁醇内标工作液加入2．0mL塑料离心管中，混合均匀，离心后将清液通过0．45μm的无机滤膜过滤后装入2．0mL进样瓶中密封，应用自动进样器进样，采用ZB-624柱分离样品中的乙醇和叔丁醇，然后采用内标一曲线法定量分析血液中的乙醇含量。结果：内标一标准曲线法定量分析血液中的乙醇含量，乙醇的加标回收率在97．6％～101．0％，相对标准偏差小于2．0％，且重现性好。结论：该法分析血液中的乙醇含量能满足检测工作要求，相较顶空进样一气相色谱法更简单，难度也更小。The objective is to establish the internal standard quantitative method and standard curve for determi- nation of alcohol content in blood by gas chromatography method, and compared with SF/Z JD0107001-2010. We use pipe to measure with 2. 0 mL plastic centrifuge tube ,0. 20 mL blood and 1.00 mL 40. 0 g/mL TERT butanol in- ternal standard working solution mixed. The supernatant after centrifugation by inorganic membrane filtration 0.45 μm is taken into 2. 0 mL sample bottle seal, the application of automatic sample, ZB-624 column separation ethanol and TERT butyl alcohol in the sample. And then the internal standard curve method is used for the quantitative a- nalysis of alcohol content in blood. Results show that by the internal standard quantitative method and standard curve analysis of alcohol content in blood ,after the recovery of ethanol at the rate of 97.6 % - 101% ,the relative standard deviation is less than 2 %, and has good repeatability. Conclusion ： Determination of ethanol content in the blood of the method can meet the detection requirement, by headspace gas chromatography method is more simplewith less difficulty.

关 键 词：内标一标准曲线法 气相色谱法 血液 乙醇 

分 类 号：DF795.1[医药卫生—法医学]