Taqman-MGB探针在毒麦鉴定上的应用  

Application of Taqman-MGB probe to identify Lolium temulentum

作　　者：吴申懋 邓志瑞[1] 陈沁[1] 傅怡宁[2] 薛华杰[2] 印丽萍[2] 

机构地区：[1]上海大学生命科学学院,上海200444 [2]上海出入境检验检疫局

出　　处：《植物检疫》2016年第1期40-43,共4页Plant Quarantine

基　　金：国家质检总局项目(2013IK281);国家质检总局项目(2012BAK11B03)

摘　　要：本研究对毒麦及其近似种的ITS2序列进行扩增测序,根据序列比对得到的多态性位点设计特异性引物及Taqman-MGB探针,建立Real-time PCR检测毒麦的方法;同时对该方法的特异性、灵敏度、重复性进行评估。结果表明:该方法检测灵敏度能达到5×10-3 ng/μL,对其他黑麦草属样品检测结果均为阴性。本研究为毒麦样品的快速、准确检测奠定了基础。The ITS2 regions of Lolium temulentum and its related species were amplified and sequenced.The probe and specific primers were designed based on SNP（Single Nucleotide Polymorphisms）. The specificity,sensitivity and reproducibility of the developed Taqman-MGB method were estimated. The results showed that this method had high repeatability and the sensitivity reached to 5 ×10-3ng/μL. The results of other Lolium samples were negative. This research laid the foundation for rapid,accurate detection of Lolium temulentum.

关 键 词：毒麦 TAQMAN-MGB探针 实时荧光PCR 

分 类 号：S452[农业科学—植物保护]