利用多重PCR诊断水稻白叶枯病和细菌性条斑病的复合发生  

Dignosis of complex infection of Xanthomanas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola by Multi-plex PCR

作　　者：李云飞[1] 陈雪娇[1] 杨雪[2] 张爱芳[2] 谷春艳[2] 高同春[2] 陈雨[2] 姚剑[1] 

机构地区：[1]安徽出入境检验检疫局,安徽合肥230022 [2]安徽省农业科学院植物保护与农产品质量安全研究所

出　　处：《植物检疫》2016年第1期48-52,共5页Plant Quarantine

基　　金：质检公益性行业专项(201410076);安徽检验检疫局项目(AHKT-01-2011);农业公益性行业专项(201303015);安徽省农科院创新团队(12C1105);安徽省水稻产业体系(AHCYTX-01)

摘　　要：为准确检测水稻白叶枯病菌、细菌性条斑病菌及这两种病菌的复合发生,利用软件DNAStar分析比较这两种菌的部分核酸序列,设计了检测这两种病菌的特异性引物。引物Xoo F-Xoo R能特异性扩增出水稻白叶枯病菌中一条大小162 bp的条带;引物Xooc F1-Xooc R1和Xooc F2-Xooc R2能够分别特异性扩增出水稻细菌性条斑病菌中690 bp和945 bp的条带。通过优化PCR反应条件,成功建立了多重PCR技术,可以对不同国家的水稻白叶枯病菌和细菌性条斑病菌进行准确检测,对由这两种病菌引起的复合侵染实现了准确诊断。In order to detect Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola and co-infection of these two pathogens,partial DNA sequences of X. oryzae pv. oryzae and X. oryzae pv. oryzicola were analyzed and the specific primers were designed by software DNAStar. A 162 bp fragment could be amplified from X. oryzae pv. oryzae with the primer pair Xoo F-Xoo R while 690 bp and 945 bp fragment could be amplified from X. oryzae pv. oryzicola with the primer pair Xooc F1-Xooc R1 and Xooc F2-Xooc R2 respectively. After optimization of the PCR condition,multi-plex PCR was successfully developed,with which could detect X. oryzae pv. oryzae and X. oryzae pv. oryzicola from different countries precisely. The result demonstrated that multi-plex PCR could detect and identify the co-infection of these two pathogens.

关 键 词：水稻白叶枯病菌 水稻细菌性条斑病菌 特异性引物 多重PCR 复合侵染 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]