化疗减毒汤对环磷酰胺所致小鼠骨髓抑制的保护作用  

Effects of Side-effect Attenuation Prescriptions on Cyclophosphamide-induced Myelosuppression in Mice

作　　者：王颖飞 龙飞[1] 唐振[1] 曹红春[1] 叶震中 侯晓利[1] 李世杰[2] 

机构地区：[1]成都中医药大学临床医学院,成都610075 [2]成都中医药大学附属医院肿瘤科,成都610075

出　　处：《四川大学学报（医学版）》2016年第2期214-216,261,共4页Journal of Sichuan University(Medical Sciences)

基　　金：四川省科技厅基金(No.2011SZ0312)资助

摘　　要：目的研究化疗减毒汤的应用对环磷酰胺造模小鼠外周血中白细胞(WBC)、血红蛋白(HGB)及血小板(PLT)水平的影响。方法实验小鼠随机分为6组:正常对照组、环磷酰胺骨髓抑制模型组(模型组)、重组人粒细胞集落刺激因子(G-CSF)组及化疗减毒汤大、中、小剂量组(实验大、中、小剂量组)。采用小鼠腹腔注射环磷酰胺建立骨髓抑制模型(40 mg/kg,1次/d,连续10d),化疗减毒汤组分别灌胃大、中、小剂量化疗减毒汤(原生药40g/kg、20g/kg、10g/kg),1次/d,连续给药2周,G-CSF组皮下注射G-CSF注射液(每次取血前3d皮下注射GCSF 50μg/kg),造模后7d、14d尾静脉取血检测WBC、HGB、PLT水平。结果给药7d后,化疗减毒汤可有效减缓环磷酰胺造模小鼠WBC、HGB、PLT水平的下降(P<0.05)。造模14d后,化疗减毒汤小剂量组小鼠血液WBC数量高于模型组(P<0.05),化疗减毒汤大、小剂量组小鼠血液HGB浓度也较模型组升高(P<0.05)。结论化疗减毒汤可以促进环磷酰胺造模小鼠外周血中白细胞、血红蛋白及血小板水平的恢复。Objective To determine the effects of Side-effect Attenuation Prescriptions on the levels of white blood cells（WBC）,hemoglobin（HGB）and platelet（PLT）in peripheral blood of mice influenced by cyclophosphamide（CTX）. Methods Mice were randomly divided into 6 groups：normal control group,myelosuppression group induced by CTX（model group）,recombinant human granulocyte colony stimulating factor（G-CSF）group,and Side-effect Attenuation Prescriptions group（experimental groups with high,middle,and low dosages）.Marrow depressed models were established by injecting CTX intraperitoneally（40mg/kg,once/d,10d）to the mice.High,middle,and low dosage experimental groups received 40g/kg,20g/kg,and 10g/kg Side-effect Attenuation Prescriptions once a day for two weeks,respectively.Mice in the G-CSF group were given G-CSF（50μg/kg）by hypodermic injection three days before blood sampling.Levels of WBC,HGB and PLT counts in peripheral bloods of the mice were detected at 7dand 14 dafter the marrow depressed models were established.Results The Side-effect Attenuation Prescriptions slowed down the decline of blood levels of WBC,HGB and PLT induced by CTX（P0.05）,and accelerated the recovery of WBC and HGB levels compared with model group at14d（P0.05）.Conclusion The Side-effect Attenuation Prescriptions can accelerate recovery of WBC,HGB and PLT in peripheral bloods of mice.

关 键 词：化疗减毒汤 环磷酰胺 骨髓抑制 白细胞 血红蛋白 血小板 

分 类 号：R285.5[医药卫生—中药学]