The Rapid Propagation and Domestication of Wild Bletilla striata  

野生白芨种苗快繁与驯化试验研究（英文）

作　　者：陈海锋[1] 徐森富[1] 陈依桃 

机构地区：[1]台州科技职业学院,浙江台州318020

出　　处：《Agricultural Science & Technology》2016年第2期289-290,322,共3页农业科学与技术（英文版）

基　　金：Supported by Students’S&T Innovation Project of Zhejiang Colleges and Universities(2015R463001)~~

摘　　要：The research explored rapid multiplication on Bletilla striata and the re- suits showed that the medium of MS＋2.5 mg/L6-BA＋0.2 mg/L NAA performed the best in induction, with induction rate at 98%; the medium with MS＋IAA2.5mg/L＋ 2.5 mg/L6-BA＋2.0 mg/L NAA was the best in terms of induction and multiplication; the medium with 1/2MS＋0.01 mg/L NAA 1＋0.01 mg/L IAA promoted rooting.The harden- ing temperature was the optimal at 23℃-28 ℃ and relative humidity of 80%-90% the best.对野生白芨进行组培快繁研究,结果表明：MS＋2.5 mg/L 6-BA＋0.2 mg/L NAA诱导效果最好,诱导率可达98%;MS＋IAA2.5mg/L＋2.5 mg/L 6-BA＋2.0 mg/L NAA诱导增殖的效果最好,培养40 d后增殖率可达3.8倍;1/2MS＋0.01 mg/L NAA1＋0.01 mg/L IAA有利于生根,且植株长势健壮。炼苗基质以60%腐叶土＋30%珍珠岩＋10%蛭石为佳;炼苗温度23~28℃,空气相对湿度80%~90%有利于提高炼苗成活率。

关 键 词：Wild Bletilla striata Tissue culture PROPAGATION DOMESTICATION 

分 类 号：S567.239[农业科学—中草药栽培]