Effects of Calcium Element on Biomass and Effective Constituents Contents in Blumea balsamifera in Slow Growth Period of Winter  

钙元素对冬季迟缓期的艾纳香生物量和有效成分含量的影响（英文）

作　　者：王丹[1,2] 马青松 范佐旺[1,2] 李小婷[1,2] 宛骏[1,2] 陈振夏 庞玉新[1,2] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所/农业部华南作物基因资源与种质创制重点开放实验室,海南儋州571737 [2]海南省艾纳香工程技术研究中心,海南儋州571737

出　　处：《Agricultural Science & Technology》2016年第2期358-361,437,共5页农业科学与技术（英文版）

基　　金：Supported by National Natural Science Foundation of China(81403035;81374065)~~

摘　　要：The one-year-old seedlings of Blumea balsamifera （L.） DC. were applied with CaCl2.H2O that supplied Ca in slow growth period of winter three times. The heights, ground diameters, leaf lengths, leaf widths and biomasses of B. balsamifera plants were measured. In addition, the relative contents of total flavones in different parts of B. balsamifera were determined by UV spectrophotometry, and the absolute contents of total flavones were calculated. The relative contents of L-borneol in leaves of B. balsamifera were determined by GC, and the absolute contents of L- borneol were calculated. The results showed that calcium element significantly in- creased the biomasses in leaves, stems and roots of B. balsamifera in slow growth period of winter. The leaf biomass of B. balsamifera in the 5 g/L CaCl2-H2O treat- ment group was significantly higher than those in the other three treatment groups. The leaf biomasses of B. balsamifera in the 10 and 15 g/L CaCl2.H2O treatment groups were significantly higher than that in the CK, with 3.03 and 2.65 times, re- spectively. The application of Ca inhibited the accumulation of total flavones relative contents, but significantly increased the total flavones absolute contents in different parts of B. balsamifera. The relative and absolute contents of L-borneol in the 5 g/L CaCl2 .H2O treatment group were 0.22% and 0.22 g, which were increased by 37.50%, 22.22%, 37.50% and 100%, 100%, 450%, respectively compared with those in the 0, 10 and 15 g/L CaCl2.H2O treatment groups. The Ca element could signifi- cantly promote the accumulation of biomasses in leaves, stems and roots, as well as the absolute contents of total flavones and L-borneol in B. balsamifera in slow growth period of winter.以一年生艾纳香种子苗为实验材料,用一水合氯化钙提供钙元素,在冬季艾纳香生长迟缓期进行3次施肥,测定艾纳香的株高、地径、叶长和叶宽等生长指标以及生物量。采用紫外可见分光光度法测定艾纳香不同部位中总黄酮的相对含量,并计算总黄酮的绝对含量;采用GC测定艾纳香叶片中l-龙脑的相对含量,并计算l-龙脑的绝对含量。结果表明:钙元素极显著增加了冬季生长迟缓期的艾纳香叶、茎和根生物量,其中5 g/L钙处理组的艾纳香叶生物量极显著高于其他3个处理组,10、15 g/L钙处理下的叶生物量极显著高于空白对照组(CK),分别是对照的3.03倍和2.65倍。钙元素的施加抑制了艾纳香不同部位总黄酮相对含量的积累,然而显著增加了总黄酮绝对含量。5 g/L钙处理组的l-龙脑相对含量和绝对含量最高,分别为0.22%和0.22 g,与10、15g/L钙和CK组相比,分别增加了37.50%、22.22%、37.50%和100%、100%、450%。在冬季艾纳香生长迟缓期施加钙元素可以显著促进艾纳香叶、茎和根生物量的积累,提高总黄酮和l-龙脑的绝对含量。

关 键 词：Calcium （Ca） Blumea balsamifera Slow growth period L-borneol Total flavones： Biomass 

分 类 号：S567.239[农业科学—中草药栽培]