津力达颗粒改善1型糖尿病大鼠肝脏损伤的疗效及可能机制  

Therapeutic effect of Jinlida granules on the liver injury of mice with type 1 diabetes mellitus and possible mechanisms

作　　者：叶菲[1] 卢晓晓 刘子毓[1] 陈海燕 石勇铨[1] 刘志民[1] 

机构地区：[1]第二军医大学附属长征医院内分泌科,上海200003

出　　处：《上海交通大学学报（医学版）》2016年第2期206-210,共5页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的探讨津力达颗粒对1型糖尿病大鼠肝脏损伤的疗效及可能机制。方法采用链脲佐菌素腹腔一次性注射诱发SD大鼠糖尿病模型。将成模大鼠随机分为糖尿病模型组(DM组)、0.75 g/kg药物组、1.5 g/kg药物组、3.0 g/kg药物组,另设正常对照组(NC组),每组10只。给药8周后测定空腹血糖(FBG)、糖化血红蛋白(Hb A1C)、胰岛素、C肽、肝功能指标、肝脏纤维化指标、氧化应激指标、炎症指标,观察肝组织病理学改变。结果 DM组与NC组比较,FBG、肝酶、肝脏纤维化指标、氧化应激指标、炎症因子水平均升高;3.0 g/kg药物组给药后谷丙转氨酶(GPT)、谷草转氨酶(GOT)、碱性磷酸酶(AKP)、透明质酸(HA)、Ⅳ型胶原(Ⅳ-C)、Ⅲ型前胶原(PC-Ⅲ)、血清层黏连蛋白(LN)水平降低,超氧化物歧化酶(SOD)、丙二醛(MAD)、一氧化氮(NO)、活性氧簇(ROS)水平降低,谷胱甘肽过氧化物酶(GSH-PX)、过氧化氢酶(CAT)水平升高;3个药物组给药后C反应蛋白(CRP)、白介素-6(IL-6)均显著降低,大鼠肝纤维化病变减轻。结论津力达颗粒对1型糖尿病大鼠的肝损伤有保护作用,其机制可能与降低氧化应激反应以及改善炎症状态有关。Objective To investigate the therapeutic effect of Jinlida granules on the liver injury of mice with type 1 diabetes mellitus and possible mechanisms.Methods The SD mouse model of type 1 diabetes mellitus was established by a single intraperitoneal injection of streptozotocin（STZ）.The model rats were randomly divided into the model group（DM group）,0.75 g/kg,1.5 g/kg,and 3.0 g/kg therapy groups.A normal control group（NC group） was established and there were 10 rats in every group.The fasting glucose（FBG）,HbA1 C,insulin,C-peptide,liver function indexes,hepatic fibrosis indexes,oxidative stress indexes,and inflammation indexes were detected and pathological changes of liver tissues were observed 8 weeks after the administration.Results Compared with the NC group,levels of FBG,liver enzyme,hepatic fibrosis indexes,oxidative stress indexes,and inflammation factors of the DM group increased.Levels of GPT,GOT,AKP,HA,IV-C,PC-Ⅲ,LN,SOD,MAD,NO,and ROS of the 3.0 g/kg therapy group decreased after administration,while levels of GSH-PX and CAT increased.Levels of C RP and IL-6 of three therapy groups decreased significantly and the hepatic fibrosis alleviated.Conclusion Jinlida granules have protective effect on the liver injury of mice with type 1 diabetes mellitus.The mechanism may be relevant to the decrease of oxidative stress reaction and alleviation of inflammation.

关 键 词：津力达颗粒 糖尿病 肝损伤 肝脏纤维化 氧化应激 炎症 

分 类 号：R285.5[医药卫生—中药学]