黄芩药材中黄芩苷的提取纯化工艺优化  

The Optimization of Extraction and Purification Process of the Baicalin in Scutellaria Baicalensis

作　　者：张琳[1] 周化更 刘沛[1] 刘喜纲[1] 常金花[1] 刘翠哲[1] 

机构地区：[1]河北省中药研究与开发重点实验室/承德医学院中药研究所,河北承德067000

出　　处：《河北医学》2016年第2期177-180,共4页Hebei Medicine

基　　金：国家自然基金;(No.81341143);河北省高校重点学科建设项目资助;河北省高等学校科学技术研究青年基金项目;(QN20131020)

摘　　要：目的:优选黄芩苷的非酸碱提取纯化工艺。方法:采用大孔吸附树脂提取纯化黄芩苷,以HPLC法测定黄芩苷的含量;以黄芩苷的含量为考察指标,采用水和50%乙醇作为提取溶剂,用AB-8型大孔吸附树脂纯化,用不同浓度的乙醇洗脱,逐步考察黄芩苷在提取、吸附、洗脱时的转移率。结果:优选的提取纯化工艺为50%乙醇提取,提取液回收乙醇,经过AB-8型大孔吸附树脂吸附,以70%乙醇洗脱。结论:本实验优选非酸碱方法提取纯化黄芩药材中的黄芩苷,减少黄芩苷的破坏,减少酸碱对环境的污染,适于工业化生产。Objective： To optimize the extraction and purification methods of baicalin from scutellaria baicalensis without using acid and alkali. Method： The content of banicalin were determined by HPLC. The content of banicalin was regarded as investigation target,distilled water and 50% ethanol were used as extracting solution,the banicalin was purified by AB- 8 macroporous adsorption resin and then eluted with different concentrations of ethanol. The transfer rate was investigated. Result： The optimal extraction and purification process： extracted by 50% ethanol,recouered by ethanol,eluted by 70% ethanol. Conclusion： The extraction and purification methods of baicalin from Scutellaria baicalensis without using acid and alkali reduce the damage of baicalin and environmental pollution by acid and alkali,which is suitable for industrialized production.

关 键 词：黄芩 黄芩苷 HPLC 大孔吸附树脂 

分 类 号：R284.2[医药卫生—中药学]