mGluRs对铝致大鼠学习记忆障碍的影响  

Effect of mG luRs on learning and memory dysfunction in rats induced by aluminum

作　　者：魏建宏[1] 牛侨[2] 

机构地区：[1]山西医科大学汾阳学院,山西汾阳032200 [2]山西医科大学

出　　处：《中国老年学杂志》2016年第5期1025-1027,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(30671777);山西省自然科学基金资助项目(2013011052-2)

摘　　要：目的观察大鼠染铝后神经行为的改变以及海马区病理组织学的变化,研究代谢性谷氨酸受体(m GluRs)对铝致大鼠学习记忆障碍的影响。方法健康SD大鼠随机分为3组,经侧脑室分别注射生理盐水、0.75%Al^(3+)和1.5%Al^(3+)溶液3μl,连续5 d,而后处死分离双侧海马,用免疫组化PAP法和Western印迹检测m GluR1、m GluR3、m GluR7的表达。结果镜下显示,随着染毒剂量的增加,大鼠海马区棕黄色阳性细胞增多;图像分析软件分析和Western印迹检测均可说明染铝组中m GluR3和m GluR7的表达明显升高(P<0.05),但m GluR1的表达没有明显变化。结论铝可致大鼠神经行为发生改变,导致学习和记忆能力障碍,且与m GluR3和m GluR7的表达升高有关。Objective To observe the effects of mice after dyed aluminum on nerve behavior and the pathological and histological changes of the hippocampus,and the effect of m GluRs on the disorder of learning and memory in mice. Methods Healthy Kunming mice were randomly divided into 3 groups,with 10 mice in each group. Saline,0.75% Al^3＋and 1.5% Al^3＋were respectively injected into lateral ventricle for five days. Then the mice were executed separation of bilateral hippocampus. The expressions of m GluRs1,m GluRs3 and m GluRs7 were detected by the immunohistochemistry PAP method and Western blot.Results The microscopic showed that with the increasing of infected dose,the number of positive orange-brown hippocampus cells increased. Image analysis software analysis and Western blot detection all showed that the expressions of m GluRs3 and m GluRs7 in aluminum group were increased significantly（ P〈0.05）,but there was no significant difference in the expression of m GluRs1. Conclusions Aluminum could cause the mice neural behavior change,learning and memory impairment,which associates with elevated expressions of m GluR3 and m GluR7.

关 键 词：MGLURS 铝 学习记忆 

分 类 号：R3[医药卫生—基础医学]