新型猪流行性腹泻的诊断控制及其病原分子特征  

Diagnosis and treatment of porcine epidemic diarrhea and molecular characteristic of the pathogen

作　　者：吴美洲[1,2] 陈芳洲[1,2] 周春陵 朱银杏 库旭钢[1,2] 刘洋[1,2] 谭鑫[1,2] 姚丽[2] 何启盖[1,2] 

机构地区：[1]华中农业大学农业微生物学国家重点实验室,湖北武汉430070 [2]华中农业大学动物医学院,湖北武汉430070

出　　处：《中国兽医科学》2016年第2期149-156,共8页Chinese Veterinary Science

基　　金：国家现代产业(生猪)技术体系资助项目(CARS-36);国家自然科学基金项目(31272572)

摘　　要：2015年10月,曾免疫过猪流行性腹泻-传染性胃肠炎-轮状病毒三联活疫苗的某规模化猪场中50多头母猪出现腹泻症状,大约有600头新生仔猪的死亡,其中1周龄发病仔猪的死亡率达到95%。主要的临床症状是消瘦、被毛粗乱无光泽、水样腹泻及呕吐。通过临床调查、病理解剖观察、抗体检测、PCR检测和Sl基因、ORF3基因序列分析,确诊该病是由猪流行性腹泻病毒(PEDV)变异毒株引起,通过对仔猪血液中针对猪流行性腹泻(PED)的IgA和IgG抗体的ELISA方法检测发现,这些样品中IgA和IgG抗体水平较高,但仍然不能提供较好的免疫保护。采取母猪紧急接种YN144变异毒株制成的疫苗,配合迅速隔离发病猪只,加强保温和消毒等综合措施,迅速控制了该病。In October 2015,porcine epidemic diarrhea outbreak on a large scale pig farm with more than 1 000 sows,and the pigs in this pig farm were previously immunized with trivalent live vaccine.More than 50 sows had severe diarrhea symptom,and there were about 600 piglets within one week dead.The mortality of the piglets within one week was as higher as 95%.The main clinical symptoms of the piglets were emaciation,watery diarrhea and vomiting.By clinical investigation,pathological examination,antibodies detection and sequencing of partial S1 and ORF3 genes,the disease was confirmed as porcine epidemic diarrhea（PED）.Although the anti PED virus Ig G and Ig A levels were high in these sick piglets,but it could not provide complete protection.To control the disease,the sows were immunized with the variant PED virus-derivated attenuated YN144 vaccine,combined with the removal of sicked pigs,disinfection,and temperature adjustment,the disease was soon controlled.

关 键 词：猪流行性腹泻 新型猪流行性腹泻病毒 诊断 控制 分子特征 

分 类 号：S852.659.6[农业科学—基础兽医学]